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I1643

Sigma-Aldrich

Pyrophosphatase, Inorganic from baker′s yeast (S. cerevisiae)

powder, ≥500 units/mg protein (E1%/280)

Synonym(s):

Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase

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500 MG
CN¥1,501.59

About This Item

CAS Number:
EC Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

CN¥1,501.59


Please contact Customer Service for Availability

form

powder

Quality Level

specific activity

≥500 units/mg protein (E1%/280)

mol wt

71 kDa

storage temp.

−20°C

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钼 foil, 8mm disks, thickness 0.020mm, annealed, 99.9%

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钼 foil, 8mm disks, thickness 0.025mm, annealed, 99.9%

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钼 foil, 8mm disks, thickness 0.015mm, annealed, 99.9%

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钼 foil, 8mm disks, thickness 0.075mm, annealed, 99.9%

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manufacturer/tradename

Goodfellow 706-539-99

manufacturer/tradename

Goodfellow 771-516-29

manufacturer/tradename

Goodfellow 884-221-27

manufacturer/tradename

Goodfellow 874-434-77

form

foil

form

foil

form

foil

form

foil

assay

99.9%

assay

99.9%

assay

≥99.9%

assay

≥99.9%

resistivity

5.0 μΩ-cm, 20°C

resistivity

5.0 μΩ-cm, 20°C

resistivity

5.0 μΩ-cm, 20°C

resistivity

5.0 μΩ-cm, 20°C

density

10.3 g/mL at 25 °C (lit.)

density

10.3 g/mL at 25 °C (lit.)

density

10.3 g/mL at 25 °C (lit.)

density

10.3 g/mL at 25 °C (lit.)

bp

4612 °C (lit.)

bp

4612 °C (lit.)

bp

4612 °C (lit.)

bp

4612 °C (lit.)

General description

Molecular Weight: 71 kDa (homodimer consisting of two equal subunits of molecular weight 32-35 kDa)
Extinction Coefficient: E1% = 14.5 (280 nm)
pI: 4.75
This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present.

Application

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi.
It plays an important role in protein, RNA, and DNA synthesis.
Pyrophosphatase has been used:
  • as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.[1]
  • as a component of PCR mixture.[2]
Pyrophosphatase has been used:
as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.
as a component of PCR mixture

Unit Definition

One unit will liberate 1.0 μmole of inorganic orthophosphate per min at pH 7.2 at 25 °C.

Physical form

Lyophilized powder containing 80-90% buffer salts

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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