I1643
Pyrophosphatase, Inorganic from baker′s yeast (S. cerevisiae)
powder, ≥500 units/mg protein (E1%/280)
Synonym(s):
Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase
Select a Size
CN¥1,501.59
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About This Item
CN¥1,501.59
form
powder
Quality Level
specific activity
≥500 units/mg protein (E1%/280)
mol wt
71 kDa
storage temp.
−20°C
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This Item | GF77151629 | GF88422127 | GF87443477 |
---|---|---|---|
manufacturer/tradename Goodfellow 706-539-99 | manufacturer/tradename Goodfellow 771-516-29 | manufacturer/tradename Goodfellow 884-221-27 | manufacturer/tradename Goodfellow 874-434-77 |
form foil | form foil | form foil | form foil |
assay 99.9% | assay 99.9% | assay ≥99.9% | assay ≥99.9% |
resistivity 5.0 μΩ-cm, 20°C | resistivity 5.0 μΩ-cm, 20°C | resistivity 5.0 μΩ-cm, 20°C | resistivity 5.0 μΩ-cm, 20°C |
density 10.3 g/mL at 25 °C (lit.) | density 10.3 g/mL at 25 °C (lit.) | density 10.3 g/mL at 25 °C (lit.) | density 10.3 g/mL at 25 °C (lit.) |
bp 4612 °C (lit.) | bp 4612 °C (lit.) | bp 4612 °C (lit.) | bp 4612 °C (lit.) |
General description
Extinction Coefficient: E1% = 14.5 (280 nm)
pI: 4.75
This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present.
Application
It plays an important role in protein, RNA, and DNA synthesis.
as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.
as a component of PCR mixture
Unit Definition
Physical form
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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