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SAB4700480

Sigma-Aldrich

Monoclonal Anti-CD45-FITC antibody produced in mouse

clone MEM-28, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Anti-LCA, Anti-PTPRC

biological source

mouse

Quality Level

100

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MEM-28, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

flow cytometry: suitable

isotype

IgG1

NCBI accession no.

NM_002838.3

UniProt accession no.

P08575

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... PTPRC(5788)

Related Categories

General description

The antibody MEM-28 reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
The cluster of differentiation 45 (CD45) gene is mapped to human chromosome 1q31.3-q32.1. It codes for a type I transmembrane protein and is expressed in nucleated hematopoietic cells along within its precursors. The protein consists of a varying extracellular domain and a highly conserved intracellular domain.

Immunogen

Human thymocytes and T lymphocytes

Application

The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.

Biochem/physiol Actions

Cluster of differentiation 45 (CD45) functions as a prototypic receptor and exhibits phosphatase activity. CD45 is implicated in the regulation of T-cell mediated B cell antigen receptor (BCR)-signaling. The encoded protein plays an essential role in antigen-stimulated proliferation of T lymphocytes and in thymic development. CD45 negatively regulates cytokine receptor signaling via suppressing JAK (Janus kinase) activity.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
含少量动物源组分生物产品

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T-helper signals restore B-cell receptor signaling in autoreactive anergic B cells by upregulating CD45 phosphatase activity.
Szodoray P
The Journal of Allergy and Clinical Immunology, 138, 839-851 (2016)
Intracellular B Lymphocyte Signalling and the Regulation of Humoral Immunity and Autoimmunity.
Taher TE
Clinical Reviews in Allergy & Immunology, 53, 237-264 (2017)
The soluble cytoplasmic tail of CD45 (ct-CD45) in human plasma contributes to keep T cells in a quiescent state.
Puck A
European Journal of Immunology, 47, 193-205 (2017)
CD45 is a JAK phosphatase and negatively regulates cytokine receptor signalling
Irie-Sasaki J
Nature, 409, 349-354 (2001)
I S Trowbridge et al.
Annual review of immunology, 12, 85-116 (1994-01-01)
CD45 is one of the most abundant leukocyte cell surface glycoproteins and is expressed exclusively upon cells of the hematopoietic system. Different isoforms of CD45 are generated by alternative splicing and are expressed in cell type-specific patterns on functional subpopulations
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