SAB4700563
Anti-Cd8a low endotoxin antibody, Rat monoclonal
clone 53-6.7, purified immunoglobulin, buffered aqueous solution
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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
53-6.7, monoclonal
Application:
flow cytometry
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
Species reactivity:
mouse
Citations:
7
Technique(s):
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
Uniprot accession no.:
Product Name
Anti-Cd8a low endotoxin antibody, Rat monoclonal, clone 53-6.7, purified immunoglobulin, buffered aqueous solution
biological source
rat
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
53-6.7, monoclonal
form
buffered aqueous solution
species reactivity
mouse
concentration
1 mg/mL
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
impurities
≤1 EU/μg endotoxin (LAL)
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Cd86(12524)
Related Categories
Application
The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1.5 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
General description
The rat monoclonal antibody 53-6.7 recognizes mouse CD8a (32-34 kDa; alpha chain of the CD8 antigen).
Immunogen
Mouse spleen cells
Physical form
Solution in azide free phosphate buffered saline, pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL
test.
test.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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M J Smyth et al.
Journal of virology, 72(7), 5948-5954 (1998-06-17)
Mouse cytotoxic T lymphocytes (CTL) reactive with a H-2Db-presented 9-mer peptide of the human papillomavirus type 16 protein E7(49-57) (RAHYNIVTF) were generated from the spleen cells of wild-type C57BL/6 (B6) or B6 perforin-deficient (B6.P0) mice. CD8(+) B6 CTL displayed peptide-specific
G Das et al.
The Journal of experimental medicine, 190(6), 881-884 (1999-09-28)
Peripheral CD8(+) T cells mainly use CD8alpha/beta, and their development is mainly dependent on the major histocompatibility complex (MHC) class I proteins K(b) and D(b) in H-2(b) mice. In this report, we have shown that the development of CD8alpha/beta TCR-alpha/beta
S Stäger et al.
Journal of immunology (Baltimore, Md. : 1950), 165(12), 7064-7071 (2000-12-20)
Vaccination against visceral leishmaniasis has received limited attention compared with cutaneous leishmaniasis, although the need for an effective vaccine against visceral leishmaniasis is pressing. In this study, we demonstrate for the first time that a recombinant stage-specific hydrophilic surface protein
Scott A Gerber et al.
International journal of cancer, 134(10), 2383-2392 (2013-10-25)
Radiation therapy (RT) continues to be a cornerstone in the treatment for many cancers. Unfortunately, not all individuals respond effectively to RT resulting clinically in two groups consisting of nonresponders (progressive disease) and responders (tumor control/cure). The mechanisms that govern
G Lertmemongkolchai et al.
Journal of immunology (Baltimore, Md. : 1950), 166(2), 1097-1105 (2001-01-06)
The bacterium Burkholderia pseudomallei causes a life-threatening disease called melioidosis. In vivo experiments in mice have identified that a rapid IFN-gamma response is essential for host survival. To identify the cellular sources of IFN-gamma, spleen cells from uninfected mice were
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