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Merck
CN

SAB4700563

Anti-Cd8a low endotoxin antibody, Rat monoclonal

clone 53-6.7, purified immunoglobulin, buffered aqueous solution

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
53-6.7, monoclonal
Application:
FACS, ICC, IHC, IP
Citations:
7
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biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

53-6.7, monoclonal

form

buffered aqueous solution

species reactivity

mouse

concentration

1 mg/mL

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

impurities

≤1 EU/μg endotoxin (LAL)

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

mouse ... Cd86(12524)

General description

The rat monoclonal antibody 53-6.7 recognizes mouse CD8a (32-34 kDa; alpha chain of the CD8 antigen).

Immunogen

Mouse spleen cells

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1.5 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in azide free phosphate buffered saline, pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL
test.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Scott A Gerber et al.
International journal of cancer, 134(10), 2383-2392 (2013-10-25)
Radiation therapy (RT) continues to be a cornerstone in the treatment for many cancers. Unfortunately, not all individuals respond effectively to RT resulting clinically in two groups consisting of nonresponders (progressive disease) and responders (tumor control/cure). The mechanisms that govern
G Das et al.
The Journal of experimental medicine, 190(6), 881-884 (1999-09-28)
Peripheral CD8(+) T cells mainly use CD8alpha/beta, and their development is mainly dependent on the major histocompatibility complex (MHC) class I proteins K(b) and D(b) in H-2(b) mice. In this report, we have shown that the development of CD8alpha/beta TCR-alpha/beta
M J Smyth et al.
Journal of virology, 72(7), 5948-5954 (1998-06-17)
Mouse cytotoxic T lymphocytes (CTL) reactive with a H-2Db-presented 9-mer peptide of the human papillomavirus type 16 protein E7(49-57) (RAHYNIVTF) were generated from the spleen cells of wild-type C57BL/6 (B6) or B6 perforin-deficient (B6.P0) mice. CD8(+) B6 CTL displayed peptide-specific



Global Trade Item Number

SKUGTIN
SAB4700563-100UG04061832715896