Anti-MT-CO2 Antibody, clone 1F12 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1F12 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Cytochrome c oxidase subunit 2 (MT-CO2). It targets an epitope within 22 amino acids from the C-terminal half.

KLH-conjugated liner peptide corresponding to 22 amino acids from the C-terminal half of human Cytochrome c oxidase subunit 2 (MT-CO2).

Quality Control Testing

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MT-CO2 in A431 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MT-CO2 in Human skeletal muscle tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected MT-CO2 in Human stomach tissue sections.

Affinity Binding Assay:: A representative lot of this antibody bound MT-CO2 peptide with a KD of 3.5 x 10-7 in an affinity binding assay.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected MT-CO2 in one million A431 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Cytochrome c oxidase subunit 2 (UniProt: P00403; also known as EC:7.1.1.9, Cytochrome c oxidase polypeptide II) is encoded by the MT-CO2 (also known as COII, COX2) gene (Gene ID: 4513) in human. Cytochrome c oxidase is the terminal complex (complex IV) of the electron transfer chain that catalyzes the transfer of electrons from ferrocytochrome c to molecular oxygen and converting oxygen to water. It consists of 13 different subunits of which three subunits MT-CO1, MT-CO2, and MT-CO3 (CoxI, II, and III) are encoded by the mitochondrial genome and ten other subunits are encoded by nuclear DNA. In its active form it is shown to be a dimer. It also contains three copper ions that are essential for its function. MT-CO2 (CoxII) is a multi-pass membrane protein that is one of the three subunits which are responsible for the formation of the functional core of the cytochrome c oxidase. It contains two mitochondrial intermembrane domains (aa 1-14 and 88-227), two transmembrane domains (aa 15-45 and 60-87) and mitochondrial matrix domain (aa 46-59). MT-CO2 contains one redox center, CuA, and collects electrons from ferrocytochrome b. The electrons are then transferred to cytochrome a of subunit I and on to the cytochrome a3-CuB binuclear reaction center. The CuA most likely resides in a loop containing conserved cysteine 196 and 200 and a conserved histidine 204. Cytochrome c is reported to interact with MT-CO2 through the association of a ring of lysines around the heme edge of cytochrome c with carboxyls in MT-CO2, specifically glutamate 129, aspartate 132, and glutamate 198. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonal. (Ref.: Li, Y., et al. (2006). J. Bioenerg. Biomembr. 38(5-6); 283-291; Richter, OMH., and Ludwig, B. (2003). Rev. Physiol. Biochem. Pharmacol. 147; 47-74; Hill, BC. (2003). J. Bioenerg. Biomembr. 25(2); 115-120).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.