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Merck
CN

67038-U

BIOshell™ A400 Protein C4 (3.4 μm) HPLC Columns

L × I.D. 5 cm × 300 μm, HPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

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Product Name

BIOshell™ A400 Protein C4, 3.4 μm HPLC Column, 3.4 μm particle size, L × I.D. 5 cm × 300 μm

operating pH range

2-9

material

stainless steel hardware

agency

suitable for USP L26

description

Shell thickness (0.2 μm)
Solid Core (3.0 μm)

feature

endcapped

packaging

1 ea of

extent of labeling

0.4% carbon loading

parameter

600 bar max. pressure
90 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

5 cm × 300 μm

surface area

15 m2/g

surface coverage

4.2 μmol/m2

matrix

spherical silica particle platform
superficially porous particle

matrix active group

C4 (butyl) phase

particle size

3.4 μm

pore size

400 Å

separation technique

reversed phase

Quality Level

100

product line

BIOshell™

manufacturer/tradename

BIOshell™

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Other Notes

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General description

BIOshell™ 400 Å Protein C4 columns contain 3.4 μm advanced Fused-Core particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns provide excellent separations on very hydrophobic proteins such as those found in the cell membrane. The C4 ligand combined with an exhaustively endcapped surface ensures optimum protein recovery of larger or very hydrophobic biomolecules. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C4 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 2 to 9.

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
USXYZ001307
USXYZ001306
USXYZ001305
USXYZ001304
USXYZ001303

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Articles

BIOshell™ A400 Protein C4 U/HPLC Columns

BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.

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Care and Use Sheet for BIOshell A400 Protein C4 Nano/Capillary Columns

Care & Use Sheet for BIOshell A400 Protein C4 Nano/Capillary Columns

Care and Use Sheet for 3.4 um BIOshell A400 Protein C4 Columns

Care & Use Sheet for 3.4 μm BIOshell A400 Protein C4 Columns

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