Endonuclease enzymes are used to reduce the level of nucleic acid contamination in bioprocessing workflows and research applications by cleaving DNA and RNA into smaller fragments. While a wide variety of natural sources are available, these endonuclease formulations can pose a challenge, however, as they may contain protease activity and viral contaminants.
Benzonase® endonuclease, a genetically engineered nuclease from Serratia marcescens, eliminates the risk of using naturally derived endonucleases and can degrade all forms of DNA and RNA. When used under appropriate reaction conditions, Benzonase® endonuclease will degrade all forms of DNA and RNA (single stranded, double stranded, linear, and circular) to oligonucleotides of approximately three to five base pairs in length. It has an exceptionally high level of specific activity without any detectable proteolytic activity.
With some manufacturing templates, use of a salt concentration above physiological levels (200mM NaCl) can be beneficial. Benzonase® Salt Tolerant endonuclease enables use of salt concentrations from 200mM to 1000mM NaCl for the midstream lysis and DNA digestion step during adeno-associated virus (AAV) vector production. This new addition to the Benzonase® portfolio is compatible with all tested detergents including Deviron® detergents, which are an alternative to Triton™ X-100.
Learn more about bioprocessing and research applications of Benzonase® endonuclease.
Benzonase® endonuclease is well-suited for stringent bioprocessing applications. It degrades nucleic acids well below the 200 base pair fragment size required by the World Health Organization (WHO) and US Food and Drug Administration (FDA), is released under GMP IPE-PQG conditions, and has a Drug Master File in place with the FDA which can be cited in regulatory filings.
It is effective across a wide range of operating conditions and applications including:
Three versions of IPEG PQG GMP Benzonase® endonuclease are available to meet different manufacturing needs. All are part of the Emprove® program that facilitates preparation of documents for regulatory submissions (Table 1).
Benzonase® endonucleases are prepared in the E. coli W3110 expression system and pose a minimal risk of viral contamination.
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Benzonase® endonuclease can be used for a variety of research applications including:
A variety of high purity, high concentration Benzonase® endonuclease options are available.
Benzonase® endonuclease is also available packaged with protein extraction reagents to further facilitate workflows.
Technical Information |
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The best-in-class solution for nucleic acid removal at high salt concentrations.
The overall process yield of adeno-associated virus (AAV) manufacturing is a major concern for biotherapeutic manufacturers
Ensuring efficiency and regulatory compliance — The smart solution for DNA removal in biopharmaceutical production
Production of adeno-associated virus (AAV) vectors includes a midstream step that combines cell lysis and nuclease treatment.
Detergent, EMPROVE® EXPERT
Optimization of Benzonase® endonuclease use in virus purification.
Benzonase® endonuclease— the smart solution for DNA removal in biopharmaceutical production has proven its value for over 30 years.
Balancing efficiency and regulatory compliance—the smart solution for DNA removal in biopharmaceutical production.
This page lists nine frequently asked questions and answers about Benzonase® Nuclease.
Whether you are developing cell and gene therapies or viral vaccines, removing contaminating nucleic acids is an important part of viral production. The simplest way to do this is to use the Benzonase® endonuclease.
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