diluted in buffer (B) to obtain
standard solutions at the following
concentrations: 500, 250, 125, 63, 31 and
15 pg/0.1 ml.
B. Dilution buffer: 0.05 M Tris-HCl (Product No.
confirmed by
5-bromo-2′-deoxyuridine incorporation in human
peripheral blood mononuclear cells at ≤125 µg/ml
culture medium.
Precautions and Disclaimer
This product is for R&D use only, not for drug
With cold pack 2-8
oC 02-0865-00 1 x 100 mL
2 Blocking Buffer With cold pack 2-8
oC 02-0866-00 1 x 250 mL
3 Plate Standard Diluent With cold pack 2-8
oC 02-0867-00 1 x 125 mL
standard solutions at the following
concentrations: 250, 125, 63, 31 and 15 pg/0.1 ml.
(B) Dilution buffer: 0.05 M Tris-HCl (Product No.
Z26,547-0), pH 8.0, containing 0.1 M NaCl, 0.1%
gelatin (No. G2500
further diluted in
buffer (B) to obtain standard solutions at the fol-
lowing concentrations: 250, 125, 63, 31 and 15
pg/0.1 ml.
(B) Dilution buffer: 0.05 M Tris-HCl (Sigma Product
No. T-3523), pH 8.0
dissolve
one tablet either in:
• 100 µL of N,N-dimethylformamide (DMF) with
a final volume of ~125 µL (80 mg/mL final
concentration)
• 180 µL of dimethyl sulfoxide (DMSO) with a
final volume of
each well of a MultiScreen Deep Well
Solvinert filter plate.
2. Aspirate 125 µL serum sample (with drug), aspirate 125 µL acetonitrile
(from the Deep Well plate), and dispense both back into the MultiScreen
following concentrations: 7.5, 15,
powder dissolves. This is the stock antiserum solution. 31, 63, 125, 250, and 500 pg/0.1 ml.
To obtain the number of tests indicated on the vial, the (B) Tris-HCL (Sigma
CHO cell suspension from the cryovial
to the 125 mL Erlenmeyer flask in order to achieve
a seeding density of 3x105 – 5x105 cells/mL, and
transfer to a 125 mL Erlenmeyer flask for cultivation.
Culture
Ergonomic design
The optimized design, the prac-
tical arrangement of functions,
and the low weight of 125 g
offer reliable operation during
serial pipetting tasks, even for
inexperienced users.
Spare
cultures including T-helper cell
clones and suboptimal doses of antigen. J. Nutr.,
125(10), 2483-92 (1995).
CMH/NSB 11/02
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc
mM
NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼125 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 48–64 nmole/min/mg (see Figure 2
(1.0 mL tube)
96 well 1–3 300–1,000
24 well 2–5 200–500
12 well 4–10 100–250
6 well 8–16 60–125
Precautions and Disclaimer
For R&D use only, not for drug, household, or other
uses. Please
contains enough reagents to enrich four compartmental proteins from 5 grams
tissues or approximately 125 million cells. The efficiency of subcellular fractionation has
been investigated by SDS-PAGE and immunoblotting