Genomics

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facet applications:Genomics

facet applications:Next Gen sequencing

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Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.

Cas9-Mediated Recombineering Enhances Metabolic Production of Kdo2-Lipid A by E. coli

In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either

Probe-based qPCR

Probe based QPCR utilizes a fluorescent–labeled target-specific probe resulting in increased specificity and sensitivity. Additionally, a variety of fluorescent dyes are available so that multiple primers can be used to simultaneously amplify many sequences.

Plasmid Product Nomenclature

The SnapFast system is a versatile plasmid cloning platform that provides a range of functional DNA sequences in an easy to clone format. Hundreds of pre-designed DNA sections that can be easily incorporated into, or transferred between, our range of

ChIC/CUT&RUN Kits

Chromatin Immunocleavage (ChIC) kits and CUT&RUN technology overview for improved chromatin isolation and downstream analyses.

Extract-N-Amp™ Plant Tissue PCR Kits

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that

Modification of the WTA2 Amplification Product for Next Generation Sequencing

Transplex Whole Transcriptome Amplification (WTA2) exponentially amplifies RNA producing a double-stranded cDNA library while precisely maintaining differential levels of individual transcripts in test and reference samples.

Kapa Biosystems qPCR Reagents and Kits

Quantitative PCR (qPCR) and qRT-PCR reagents and kits that are designed to deliver maximum efficiency and reliable results from a variety of genetic materials.

ChIC and CUT&RUN Data Analysis Tool Overview

Chromatin Immunocleavage (ChIC) and CUT&RUN data analysis tool for file management and downstream sequencing data analyses.

Benzonase^® Nuclease for Microbiome Workflows

Benzonase® Nuclease for reducing host DNA in microbiome workflows and enhancing taxa identification.

MetaPolyzyme, DNA Free Cell Lysis Enzymes for Microbiome Workflows | Sigma-Aldrich

Explore the benefits of MetaPolyzyme, DNA free to improve DNA extraction and increase the number of taxa identified in treated samples.

Impact of GenElute™-E Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes

Impact of Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes. Evaluation of the purity of genomic DNA by UV spectrophotometry, gel electrophoresis, and downstream qPCR using GenElute™-E DNA purification kits.

Positive Control siRNA

MISSION Positive Control siRNA, designed using the Rosetta siRNA Design Algorithm, serve as ideal complement to any siRNA silencing experiment. All MISSION Positive Control siRNA have been validated to work, so that you can spend more time silencing your gene

Custom Oligos Modifications

We offer over 200 custom DNA oligos modification for both DNA, RNA oligonucleotides and sequencing to probes for gene.

ChIC and CUT&RUN Data Analysis Tool Frequently Asked Questions (FAQs)

Chromatin Immunocleavage (ChIC) and CUT&RUN data analysis tool frequently asked questions (FAQs).

KAPA Express Extract Kit FAQs

Extract DNA from crude samples for PCR applications, including qPCR, using the KAPA Express Extract Kit. See frequently asked questions regarding sample type, optimization and storage.

Water for pH Measurement and Buffer Preparation

Learn how water impurities can affect pH. Water quality defined as pure water or Type 2 water is recommended to prepare buffer solutions or measure pH.

siRNA Universal Negative Controls

MISSION siRNA Universal Negative Controls are an essential component to any siRNA experiment. Using a Negative siRNA control allows the researcher to create a baseline for mRNA knockdown efficiency. MISSION siRNA Universal Negative Controls have been tested in human, rat

MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing

Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.

DNA Methylation and Bisulfite Conversion

There are several common ways to determine whether a gene contains methylated DNA. Since mammalian methylation occurs at cytosines, researchers take advantage of the fact that methylated cytosine (meC) is stable to bisulfite treatment but unmethylated cytosine is transformed to

Whole Transcriptome Amplification of RNA from Low Cell-Number Samples

The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.

Genomic DNA Preparation Troubleshooting

Description of common problems that can occur during genomic DNA preparation

Water for Next-Generation Sequencing

Next-generation sequencing (NGS) revolutionized genomic research, and is now playing a crucial role in the clinical environment.

DNA Standards for Metagenomic Research into Microbiome Communities

The use of standards is critical to the integrity of metagenomics research. Learn how DNA standards for bacteria, fungi, and viruses are applied to studying the microbiome. Choose standards for E. coli and other key species, as well as mixed

Colony PCR

Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.

SeqPlex™-I Whole Transcriptome Amplification Kits for Next-Generation Sequencing

Whole transcriptome amplification (WTA) is a method often used to amplify small quantities of reverse transcribed RNA, or degraded RNA for direct use in Next-Generation Sequencing (NGS) workflows. Discover our SeqPlex™-I WTA library preparation kit for producing libraries that are

Nuclease-Free Water at Your Fingertips

An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.

Whole Genome Amplification (WGA) for Single Cell Biology

Advances in single-cell WGA have enabled the contribution of genomics to single-cell biology. Whole-genome amplification (WGA) is described as a non-specific amplification that affords a product completely representative of the initial starting material.

Minimizing Dimerization of Next-Generation Sequencing Adapters

Turn to our Next-Gen Sequencing Oligos (NGSO) as a potential solution if you are experiencing high levels of adapter dimer formation with custom adapters sourced from other vendors.

Custom Next-Gen Sequencing Oligos

Regardless of next-generation sequencing (NGS) platform, universal and index adapter sequences are required for the proper assembly of sample fragments.

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