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Extraction and Analysis of Lasix (Furosemide) in Horse Serum

Studies have suggested that there is a correlation between furosemide and improved racing performance, prompting the horse racing industry to monitor the use of this drug

Developing a Quantitative SPME Method

Quantitative NMR for Content Assignment of Reference Standards for Quality Control of Herbal Medicinal Products

qNMR has proven to be appropriate as a primary analytical method for the certification of natural products as reference standards for QC of herbal medicinals.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

New EN ISO 22964 for Cronobacter spp. detection in the food chain

The new EN ISO 22964:2017 standard specifies a horizontal method for the detection of Cronobacter spp. in food, animal feed and environmental samples.

How to use Scepter™ 2.0 Cell Counter

The Scepter™ cell counter uses the Coulter principle of impedance-based particle detection to reliably and accurately count every cell in your sample.

Selective Extraction and Cleanup of Patulin Mycotoxin from Apple Juice Samples

This selective and fast MIP extraction procedure allows trace amounts of patulin to be easily detected in apple juice, with high analyte recovery and reproducibility.

Analytical Method: Nitrite in potato instant powder

Photometric determination using the Griess method subsequent to extraction

Protocol for Preparation and Cultivation of PromoCell® Cryopreserved and Proliferating Cells

Protocol for the seeding and cultivation of primary cells received in cryopreserved or proliferating formats. Information on primary cell culture, cell handling and passaging.

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

Mouse Embryonic Stem Cell Culture Procedures & Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

Human Aortic Endothelial Cells (HAOEC) Culture Protocol

Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.

Two solvents (Dioxane, DMAc), Strong Base (NaOt-Bu) Step-by-Step Guide for Buchwald-Hartwig Amination Reaction Screening Kit

Protocol for the KitAlysis™ Buchwald-Hartwig Amination Reaction Screening Kit

Procedure for Preparation of Cationic Lipid/Oligomer Complex - Avanti® Polar Lipids

This procedure describes the preparation of Cationic Lipid/Oligomer Complex.

Kidney Transporter Assays

The kidney transporter assays provide a means to assess whether a test compound is a potential substrate or inhibitor of specific drug transporters within the kidney. We provide kidney transporter assays for small molecule formulations such as pharmaceuticals, industrial chemicals

Preparation of Cationic Liposomes & Transfection of Cells - Avanti® Polar Lipids

This procedure describes the Preparation of Cationic Liposomes & Transfection of Cells

Forskolin-Induced Swelling Assays

A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue.

Preparation of Cationic Liposomes & Transfection of Cells - Avanti® Polar Lipids

This procedure describes the Preparation of Cationic Liposomes & Transfection of Cells

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

Yeast Transformation Kit

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

Cultrex^® 3-D Culture Matrix™ Laminin I Protocol

3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro.

Derivatization of Fatty acids to FAMEs

Explore how GC can be used to analyze fatty acids either as free fatty acids or as fatty acid methyl esters.

Recombinant eSpCas9 Protein for RNP-Based Genome Editing

The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system was discovered in bacteria, where it functions as an adaptive immune system against invading viral and plasmid DNA.

Deoxyribonuclease I

Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.

Plant DNA Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.

Enzymatic Assay of Cellulase

To standardize an enzymatic assay procedure of cellulase.

Enzymatic Assay of Ficin (EC 3.4.22.3)

This procedure may be used for all Ficin products.

Enzymatic Method for Determining Alanine (Alanine Assay)

Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.

Enzymatic Assay of Fumarase

Enzymatic Assay of Superoxide Dismutase

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