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Large molecule HPLC
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Showing 1-30 of 77 results
Analytical Size-exclusion Chromatography Columns for Biomolecule Separations
Informative article on size exclusion chromatography columns for biomolecule separations. Using columns packed with sub-2 μm particles provides significant gains in efficiency over SEC columns packed with larger particles.
HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
HPLC Analysis of Sugars on Ascentis® Si
Separation of Sucrose; Maltose; Maltotriose
HPLC Analysis of Caseins on BIOshell™ A400 Protein C4
-casein basis (electrophoresis), lyophilized powder; β-Casein from bovine milk, BioUltra, ≥98% (PAGE)
Ascentis® Express Phenyl Hexyl Columns for U/HPLC
Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.
An LC/MS Peptide Standard for Rapid System Suitability Assessment and Retention Time Translation Across LC/MS Platforms
Standards are critical in liquid chromatography/mass spectrometry (LC/MS) based proteomics to ensure optimal and consistent system performance before, during, and after sample analysis.
Multiplex Quantification of Infliximab and Adalimumab in Human Serum by LC-MS/MS Using Full-Length Stable Isotope Labeled Internal Standards
Infliximab and Adalimumab monoclonal antibodies (mAbs) are used widely to treat rheumatoid arthritis, psoriatic arthritis, and many autoimmune diseases by binding to tumor necrosis factor-alpha (TNFα) to reduce the inflammatory response.
Absolute Quantification of Serum Proteome
Absolute Quantification (Protein-AQUA™™) is a targeted quantitative proteomics technique that exhibits robust efficacy and is being increasingly utilized for a wide variety of quantitative proteomics studies.
BIOshell™ A400 Protein C4 U/HPLC Columns
BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.
Rapid Trypsin Digestion of Complex Protein Mixtures for Proteomics Analysis
Reliable and reproducible results in mass spectrometry proteomics analyses are highly dependent on robust sample preparation.
How to Select the Right Water Purification System for Your Lab
Technical article on Selecting the Right Water Purification System
Developing HPLC Methods
We recommend every HPLC method developer have these four columns in their arsenal; Ascentis C18, Ascentis RP-Amide, Ascentis Phenyl and Discovery HS F5.
New Certified Reference Materials for α‑Lactalbumin, β‑Lactoglobulin A & B, and Lactoferrin
We have developed Certified Reference Materials (CRMs) of fully intact milk proteins that allow accurate testing of the most critical proteins in infant formula; help labs to develop and test infant formula products with high accuracy, while ensuring legal and
Ascentis® Express RP-Amide HPLC Columns
Sigma-Aldrich.com presents an article about innovative particle technology and RP-Amide bonding chemistry provide the benefits of speed, resolution, selectivity, aqueous stability, and improved peak shape for bases.
UHPLC Analysis of Paraquat and Diquat on Supel™ Carbon LC
Ultra-high performance liquid chromatographic (UHPLC) separation of polar herbicides, paraquat and diquat using SupelTM Carbon LC column with baseline resolution, excellent peak shape, and good lot-to-lot reproducibility.
Biopolymer Separations
Supelco’s product offering for biopolymer separations includes columns and media categorized by separation mode, as well as by column brand.
HPLC Tips & Tricks: Mobile Phase Preparation Part 2 - Buffers
Learn the tips and tricks of selecting and preparing a suitable mobile phase buffer for your HPLC separations and method developments for samples that contain ionizable compounds.
Difluoroacetic Acid as an Effective Mobile Phase Modifier for the LC-UV/MS Analysis of Proteins
The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.
Peptide Sample Amount Determination
Determining the amount of desired product in a peptide sample.
HPLC Tips & Tricks: Increase Your HPLC/UHPLC Method Sensitivity
Tips & tricks to improve your chromatographic method sensitivity by reducing baseline noise and/or making suggested improvements to increase signal intensity.
HPLC Tips & Tricks: Optimizing Injection Volume
Learn the tips and tricks to optimize the injection volume during your High-Performance Liquid Chromatography (HPLC) method-based analyses and further, how it affects your peak area.
Released N-Glycan Analysis of a Therapeutic Antibody Using BIOshell™ Glycan Column
This application note describes the released N-Glycan analysis of a monoclonal antibody, cetuximab, labeled with procainamide, using a BIOshell™ Glycan HPLC column.
Workflow for Antibody Aggregates and Fragments Analysis
A complete SEC-UV workflow for the characterization of mAb monomers, aggregates, and fragments using Zenix® and Zenix®-C SEC columns, including system suitability testing and forced pH and temperature stress studies.
UHPLC-MS Bottom-Up Analysis of Trastuzumab on a BIOshell™A160 Peptide C18 Column
Learn about UHPLC-MS analysis of trastuzumab on a BIOshell™ 160A peptide C18 column and discover reduced solvent consumption in peptide mapping using 1.5 mm I.D. columns.
Analysis of Oligonucleotide Standard 6 Mix by Liquid Chromatography-UV
Optimized separation of Oligo Standard 6, an internally created HPLC-UV system suitability mix, on Chromolith® RP-18e columns with an evaluation of the effect of flow rates up to 3 mL/min and ion-pairing reagent, TEAA, on the separation.
Monolithic Silica with Bimodal Pore Structure
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
UHPLC Analysis of Nucleosides on Supel™ Carbon LC Column: Performance Comparisons with Competitor
Ultra-high performance liquid chromatographic (UHPLC) separation of 12 nucleosides using Supel™ Carbon LC column in 15 minutes for use in identification and quantitation of nucleoside biomarkers.
Workflow for Cannabinoids Analysis in Cannabis Using Chromolith® Monolithic Silica HPLC Column Providing Low Backpressure and Extended Column Lifetime
A comprehensive high performance liquid chromatography-diode array detection (HPLC-DAD) workflow for the analysis of 14 cannabinoids in hemp bud extracts within 10 minutes using robust Chromolith® monolithic silica HPLC columns with low column back pressure.
Purospher™ STAR HPLC and UHPLC Columns
For outstanding separations with excellent peak symmetry, our range of Purospher™ STAR HPLC and UHPLC columns are available with a variety of modifications to suit your selectivity needs.
UHPLC/MS Analysis of Diazepam and Metabolites on Titan™ C18, Comparison of Flow Rate and Column Length
Separation of Oxazepam; Desmethyldiazepam, analytical standard; Temazepam, analytical standard; Diazepam
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