- Direct determination of the redox status of cysteine residues in proteins in vivo.
Direct determination of the redox status of cysteine residues in proteins in vivo.
Biochemical and biophysical research communications (2014-12-02)
Satoshi Hara, Yuki Tatenaka, Yuya Ohuchi, Toru Hisabori
PMID25436431
ABSTRACT
The redox states of proteins in cells are key factors in many cellular processes. To determine the redox status of cysteinyl thiol groups in proteins in vivo, we developed a new maleimide reagent, a photocleavable maleimide-conjugated single stranded DNA (DNA-PCMal). The DNA moiety of DNA-PCMal is easily removed by UV-irradiation, allowing DNA-PCMal to be used in Western blotting applications. Thereby the state of thiol groups in intracellular proteins can be directly evaluated. This new maleimide compound can provide information concerning redox proteins in vivo, which is important for our understanding of redox networks in the cell.
MATERIALS
Product Number
Brand
Product Description
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