Composition controlled synthesis of PCL-PEG Janus nanoparticles: magnetite nanoparticles prepared from one-pot photo-click reaction.

Composition controlled synthesis of PCL-PEG Janus nanoparticles: magnetite nanoparticles prepared from one-pot photo-click reaction.

Nanoscale (2015-02-11)

S Khoee, Y Bagheri, A Hashemi

ABSTRACT

The aim of this study is to investigate the effect of polymer nature on the morphology of synthesized nanoparticles. Super paramagnetic iron oxide nanoparticles (SPIONs) were prepared by co-precipitation method and then reacted with (3-mercaptopropyl) trimethoxysilane to obtain thiol-decorated SPIONs. Acrylated poly(caprolactone) and methoxy poly(ethylene glycol) were prepared, and then "thiol-ene click" reaction was performed under UV irradiation to attach two types of polymers on the surface of magnetite nanoparticles via the "photo-click" reaction method. Computational modelling was used for the prediction of the self-assembly of polymers on the surface of SPIONs, which determines the morphology of polymer coated nanoparticles.

MATERIALS

Product Number

Brand

Product Description

494488

Sigma-Aldrich

N,N-Dimethylformamide, biotech. grade, ≥99.9%

270547

Sigma-Aldrich

N,N-Dimethylformamide, suitable for HPLC, ≥99.9%

T0886

Sigma-Aldrich

Triethylamine, ≥99%

606758

Sigma-Aldrich

Formaldehyde-¹²C solution, 20% in H₂O, 99.9 atom % ¹²C

47673

Sigma-Aldrich

Formaldehyde solution, tested according to Ph. Eur.

PHR1553

Supelco

Dimethylformamide, Pharmaceutical Secondary Standard; Certified Reference Material

73487

Supelco

Triethylamine, analytical standard

F1635

SAFC

Formaldehyde solution, contains 10-15% methanol as stabilizer, 37 wt. % in H₂O

07102

Sigma-Aldrich

Hydrochloric acid, meets analytical specification of Ph. Eur., BP, NF, fuming, 36.5-38%

319937

Sigma-Aldrich

N,N-Dimethylformamide, ACS reagent, ≥99.8%

30721

Sigma-Aldrich

Hydrochloric acid, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., fuming, ≥37%, APHA: ≤10

258148

Sigma-Aldrich

Hydrochloric acid, ACS reagent, 37%

33120

Sigma-Aldrich

N,N-Dimethylformamide, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.8% (GC)

V900876

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Trypan Blue, Vetec^™, reagent grade

D158550

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N,N-Dimethylformamide, ReagentPlus^®, ≥99%

252549

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Formaldehyde solution, ACS reagent, 37 wt. % in H₂O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

90340

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Triethylamine, puriss. p.a., ≥99.5% (GC)

90338

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Triethylamine, for protein sequence analysis, ampule, ≥99.5% (GC)

90337

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Triethylamine, for amino acid analysis, ≥99.5% (GC)

90335

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Triethylamine, BioUltra, ≥99.5% (GC)

15512

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Formaldehyde solution, meets analytical specification of USP, ≥34.5 wt. %

93595

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Trypan Blue solution, 0.4%, for microscopy

47608

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Formaldehyde solution, for molecular biology, BioReagent, ≥36.0% in H₂O (T)

471283

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Triethylamine, ≥99.5%

227056

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N,N-Dimethylformamide, anhydrous, 99.8%

302643

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Trypan Blue, ≥80% (HPLC), Dye content 60 %

D4551

Sigma-Aldrich

N,N-Dimethylformamide, for molecular biology, ≥99%

T6146

Sigma-Aldrich

Trypan Blue, powder, BioReagent, suitable for cell culture

47083-U

Supelco

Formaldehyde solution, stabilized with methanol, ~37 wt. % in H₂O, certified reference material

72438

Supelco

N,N-Dimethylformamide, analytical standard