AS2077715: a novel antifungal antibiotic produced by Capnodium sp. 339855.

AS2077715: a novel antifungal antibiotic produced by Capnodium sp. 339855.

The Journal of antibiotics (2014-05-29)

Keisuke Ohsumi, Teruhisa Masaki, Shigehiro Takase, Masato Watanabe, Akihiko Fujie

ABSTRACT

A novel antifungal agent, AS2077715, was isolated from the fermentation broth of a fungal strain (339855) identified as a new Capnodium species based on morphological characteristics and large-subunit ribosomal DNA sequencing. AS2077715 was isolated as a white powder via solvent extraction, HP-20 and ODS-B column chromatography and crystallization, and was determined to have the molecular formula C25H41NO7. AS2077715 has a structure related to that of funiculosin, an inhibitor of mitochondrial cytochrome bc1 complex (complex III), and showed antifungal activity against Trichophyton species.

MATERIALS

Product Number

Brand

Product Description

V900090

Sigma-Aldrich

Dimethyl sulfoxide, Vetec^™, reagent grade, 99%

D5879

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Dimethyl sulfoxide, ReagentPlus^®, ≥99.5%

41639

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C7559

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Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer

D1435

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D2650

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D9170

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D4540

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D8418

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D2438

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372978

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Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%

94563

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276855

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288306

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01934

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633240

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1211006

USP

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D2050000

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PHR1320

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721956

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C2432

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Chloroform, contains 100-200 ppm amylenes as stabilizer, ≥99.5%

319988

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472301

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366919

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34943

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41640

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34869

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02487

Supelco

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