AS2077715: a novel antifungal antibiotic produced by Capnodium sp. 339855.

AS2077715: a novel antifungal antibiotic produced by Capnodium sp. 339855.

The Journal of antibiotics (2014-05-29)

Keisuke Ohsumi, Teruhisa Masaki, Shigehiro Takase, Masato Watanabe, Akihiko Fujie

ABSTRACT

A novel antifungal agent, AS2077715, was isolated from the fermentation broth of a fungal strain (339855) identified as a new Capnodium species based on morphological characteristics and large-subunit ribosomal DNA sequencing. AS2077715 was isolated as a white powder via solvent extraction, HP-20 and ODS-B column chromatography and crystallization, and was determined to have the molecular formula C25H41NO7. AS2077715 has a structure related to that of funiculosin, an inhibitor of mitochondrial cytochrome bc1 complex (complex III), and showed antifungal activity against Trichophyton species.

MATERIALS

Product Number

Brand

Product Description

717975

Sigma-Aldrich

Methanol, suitable for NMR (reference standard)

288306

Sigma-Aldrich

Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer

322415

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Methanol, anhydrous, 99.8%

644374

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Methanol, purification grade, 99.8%

C7559

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179957

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Methanol, Laboratory Reagent, ≥99.6%

472476

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Chloroform, contains amylenes as stabilizer, ACS reagent, ≥99.8%

02487

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82762

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PHR1320

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372978

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Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%

676780

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Methanol, ACS reagent, ≥99.8%

154903

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Methanol, ACS spectrophotometric grade, ≥99.9%

179337

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Methanol, ACS reagent, ≥99.8%

PHR1372

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PHR1551

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Acetonitrile(Neat), Pharmaceutical Secondary Standard; Certified Reference Material

PHR1552

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319988

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Chloroform, contains ethanol as stabilizer, ACS reagent, ≥99.8%

24229

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32213

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320390

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Methanol, ACS reagent, ≥99.8%

366919

Sigma-Aldrich

Chloroform, ACS spectrophotometric grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

C2432

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Chloroform, contains 100-200 ppm amylenes as stabilizer, ≥99.5%

494437

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439193

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646377

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