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  • Phenolic composition and antioxidant capacity of pomaces from four grape varieties (Vitis vinifera L.).

Phenolic composition and antioxidant capacity of pomaces from four grape varieties (Vitis vinifera L.).

Journal of the science of food and agriculture (2014-08-02)
Aarón de la Cerda-Carrasco, Remigio López-Solís, Hugo Nuñez-Kalasic, Álvaro Peña-Neira, Elías Obreque-Slier
ABSTRACT

Phenolic compounds are widely distributed secondary metabolites in plants usually conferring them with unique taste, flavour and health-promoting properties. In fruits of Vitis vinifera L., phenolic composition is highly dependent on grape variety. Differential extraction of these compounds from grapes during winemaking is critically associated with wine quality. By-products of winemaking, such as grape pomace, can contain significant amounts of polyphenols. However, information concerning the varietal effect on wine grape pomace is scarce. In this study, pomaces from Sauvignon Blanc (SB), Chardonnay (CH), Cabernet Sauvignon (CS) and Carménère (CA) grape varieties were characterized spectroscopically and by HPLC-DAD analysis. White grape pomaces (SB and CH) presented higher antioxidant capacities and higher contents of total phenols and total proanthocyanidins compared with red grape pomaces (CS and CA), whereas the latter showed much higher anthocyanin levels and colour intensities. Concentrations of monomeric proanthocyanidins and low-molecular-weight phenols in the four grape pomace varieties were significantly different. Grape pomaces from four varieties showed high but diverse contents of polyphenols and antioxidant capacities. Thus grape pomaces represent an important potential source of polyphenols, which could be useful for nutritional and/or pharmacological purposes.

MATERIALS
Product Number
Brand
Product Description

Supelco
Mettler-Toledo Calibration substance ME 51143093, Vanillin, traceable to primary standards (LGC)
Sigma-Aldrich
Vanillic acid, ≥97%, FG
Sigma-Aldrich
(−)-Epicatechin gallate, ≥98% (HPLC), from green tea
Supelco
Kaempferol, analytical standard
Supelco
Acetonitrile(Neat), Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Kaempferol, ≥90% (HPLC), powder
Sigma-Aldrich
Kaempferol, ≥97.0% (HPLC)
Supelco
Vanillin melting point standard, Pharmaceutical Secondary Standard; Certified Reference Material
USP
Vanillin Melting Point Standard, United States Pharmacopeia (USP) Reference Standard
Supelco
Vanillin, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Vanillic acid, purum, ≥97.0% (HPLC)
Sigma-Aldrich
Vanillic acid, 97%
Sigma-Aldrich
Vanillin, tested according to Ph. Eur.
Supelco
Vanillic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Supelco
Vanillin, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Vanillin, ReagentPlus®, 99%
Supelco
Residual Solvent - Acetonitrile(solution in DMSO), Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Quercetin, ≥95% (HPLC), solid
Sigma-Aldrich
Vanillin, ≥97%, FCC, FG
Sigma-Aldrich
Vanillin, natural, ≥97%, FCC, FG
Vanillin, European Pharmacopoeia (EP) Reference Standard
Supelco
Quercetin, Pharmaceutical Secondary Standard; Certified Reference Material
USP
Quercetin, United States Pharmacopeia (USP) Reference Standard
Supelco
Ethyl acetate, analytical standard
Sigma-Aldrich
(+)-Catechin hydrate, ≥98% (HPLC), powder
Supelco
Myricetin, analytical standard
Supelco
Gallic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
Acetonitrile, ReagentPlus®, 99%
Sigma-Aldrich
Acetonitrile, ACS reagent, ≥99.5%