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Rapid and mild purification method for nanoparticles from a dispersed solution using a monolithic silica disk.

Rapid and mild purification method for nanoparticles from a dispersed solution using a monolithic silica disk.

Journal of chromatography. A (2015-06-11)

Naoki Itoh, Tomofumi Santa, Masaru Kato

ABSTRACT

A rapid and mild purification method for nanoparticles using the commercially available monolithic silica disk, MonoSpin(®), was developed. The nanoparticles were purified from a dispersed solution by filtration with the aid of centrifugation at 2290×g for 2min. The purification conditions were rapid, mild, and simple compared with those of the conventional purification methods such as ultracentrifugation, dialysis, size exclusion chromatography, and ultrafiltration. The method was shown to be applicable for the purification of various nanoparticles, regardless of their size (from 21 to 100nm), composition material (silica, polyethylene glycol, and pegylated liposome), and encapsulated molecule (rhodamine 110 and doxorubicin). It was shown that this method is applicable to the purification of a wide range of nanoparticles in many different fields.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Methanol, anhydrous, 99.8%

Sigma-Aldrich

Triethylamine, puriss. p.a., ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, for amino acid analysis, ≥99.5% (GC)

Sigma-Aldrich

Hydrochloric acid solution, for amino acid analysis, ~6 M in H₂O

Sigma-Aldrich

Acetic acid, ≥99.5%, FCC, FG

Sigma-Aldrich

Acetic acid, natural, ≥99.5%, FG

Sigma-Aldrich

Cyclohexane, anhydrous, 99.5%

Sigma-Aldrich

Acetic acid, suitable for luminescence, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, ampule, ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Methanol, suitable for NMR (reference standard)

Sigma-Aldrich

Acetic acid-¹²C₂, 99.9 atom % ¹²C

Sigma-Aldrich

Triethylamine, ≥99.5%

Sigma-Aldrich

Triethylamine, ≥99%

Sigma-Aldrich

Adenosine 5′-phosphosulfate sodium salt, ≥85%

Sigma-Aldrich

Hydrochloric acid, 36.5-38.0%, BioReagent, Molecular Biology

Sigma-Aldrich

Hydrochloric acid solution, 1.0 N, BioReagent, suitable for cell culture

Supelco

Hydrochloric acid solution, volumetric, 0.1 M HCl (0.1N), endotoxin free

Sigma-Aldrich

Doxorubicin hydrochloride, 98.0-102.0% (HPLC)

Sigma-Aldrich

Methanol, purification grade, 99.8%

Sigma-Aldrich

Hydrochloric acid solution, 32 wt. % in H₂O, FCC

Sigma-Aldrich

N,N,N′,N′-Tetramethylethylenediamine, ≥99.5%, purified by redistillation

Sigma-Aldrich

L-Arginine, 99%, FCC, FG

SAFC

L-Arginine

Sigma-Aldrich

L-Arginine, BioUltra, ≥99.5% (NT)

Sigma-Aldrich

Ammonium persulfate, BioUltra, ≥98.0% (RT)

Sigma-Aldrich

Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

Sigma-Aldrich

N,N,N′,N′-Tetramethylethylenediamine, ReagentPlus^®, 99%

Sigma-Aldrich

Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer

Sigma-Aldrich

Acetonitrile, anhydrous, 99.8%