GC Analysis of a 37-Component FAME Mix on SP^®-2560

column

SP-2560, 100 m x 0.25 mm I.D., 0.20 μm (24056)

oven

140 °C (5 min), 8 °C/min to 180 °C, 4 °C/min to 210 °C, 20 °C/min to 250 °C (7 min)

inj. temp.

250 °C

detector

FID, 250 °C

carrier gas

hydrogen, 40 cm/sec

injection

1 μL, 100:1 split

liner

4 mm I.D., cup design

sample

Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride