GC Analysis of Parabens in Waste Water Effluent on SLB®-5ms after SPME using 85 μm Polyacrylate Fiber
Materials
related product
Headspace vial, screw top, rounded bottom (vial only)
volume 10 mL, clear glass vial, thread for 18, O.D. × H 22.5 mm × 46 mm, pkg of 100 eaMagnetic Screw Cap for Headspace Vials, 18 mm thread
PTFE/silicone septum (white PTFE/tranparent blue silicone), septum thickness 1.3 mm, pkg of 100 eaused together
SPME Fiber Assembly
85 μm Polyacrylate, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, white hubCONDITIONS
sample preparation
SPME (Solid Phase Microextraction)
sample/matrix
7 mL water + 1.75 gm NaCl in 10 mL vial spiked with parabens at 1 ug/L
SPME fiber
SPME Assy, 85 μm Polyacrylate FS, 23 ga, AUTO (White), pk/3 (57294-U)
extraction
immersion, 35 min
desorption process
270 °C 2 min
column
SLB-5ms, 20 m x 0.18 mm I.D., 0.36 μm (28576-U)
oven
65 °C (1 min), 20°C/min. to 220 °C (10 min)
inj. temp.
270 °C
detector
MSD
MSD interface
300 °C
scan range
SIM
carrier gas
helium, 1 mL/min constant
liner
0.75 m ID SPME
Description
Analysis Note
Parabens are alkyl benzoates that are common anti-microbial additives to personal care products such as lotions, deodorants, and shampoos.They are also used in some pharmaceutical preparations and food products. This has resulted in widespread exposure to these chemicals, especially through use of cosmetic products (1). Historically, these compounds have been considered “safe” however there is some controversy surrounding their use, after the results of some studies which have found some parabens to exhibit weak estrogenic effects (2). These compounds can enter the environment through wastewater discharges, and studies have found them at ppb levels in river sediments and sewage sludge, and at ppt levels in treated waste water and river water (2,3,4). A common approach for low level analysis of parabens is SPE followed by HPLC. Methods using GC for analysis typically include a derivatization step (3,4). In this application, SPME and direct GC-MS/SIM analysis without derivatization was used in the analysis of parabens spiked into treated wastewater effluent at 1 ppb.
Legal Information
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany