Analysis of Pesticides in Black Tea by GC/MS/MS on SLB^® Using Supelclean^™ Ultra Cleanup

extraction tube

Supel^™ Que non-buffered extraction tube (55294-U)

sample preparation

sample pretreatment: 1 gm sample in 50 mL centrifuge tube, add 10 mL water

sample preparation

Add QC check solution to each extract prior to GC/MS/MS analysis.

clean-up tube

Supelclean^™ Ultra 2400, 3 mL (54281-U)

clean-up process

Add approx. 100 mg of annhydrous sodium sulfate to top of Ultra cartridge. Condition cartridge with 3 mL of acetonitrile. 0.100 mL (Load 0.750 mL of sample extract. Elute with 8 mL of acetontrile containing 0.5% formic acid. Collect eluent and concentrate at 40 °C to a final volume of )

column

SLB^®-5ms, 30 m x 0.25 mm I.D., 0.250 μm (58471-U)

oven

105 °C (3 min), 10 °C/min to 130 °C, 4 °C/min to 200 °C, 8 °C/min. to 300 °C (10 min)

inj. temp.

programmed: 100°C (.05 min), 720 °C/min to 280 °C (45 min)

detector

MRM

MSD interface

300 °C

scan range

MRM