5 g multifloral honey + 5 mL bidistilled water in a 20 mL vial, allowed to equilibrate at 50 °C for 15 min with magnetic stirring
2 cm 50/30 μm divinylbenzene/carboxen on polydimethylsiloxane (57348-U)
headspace, 50 °C for 40 min with magnetic stirring
250 °C for 2 min
SUPELCOWAX 10, 30 m x 0.25 mm I.D., 0.25 μm (24079)
35 °C (8 min), 6 °C/min to 60 °C, 4 °C/min to 160 °C, 20 °C/min to 200 °C (1 min)
250 °C
230 °C
35-300 m/z
helium, 1 mL/min
0.75 mm I.D., SPME
(Honey)
In this application, headspace SPME (HS-SPME) using a Carboxen/PDMS/DVB fiber coupled with gas chromatography-mass spectrometry (GC/MS) was used for the characterization of the volatile fraction of honey samples. Using SPME it was possible to obtain and distinguish the chemical fingerprints of different types of honey. The relatively polar SUPELCOWAX 10 GC column provides excellent separation of these polar compounds.
SUPELCOWAX is a trademark of Sigma-Aldrich Co. LLC