HPLC Analysis of Amphetamine and Methamphetamine Enantiomers on Astec® CHIROBIOTIC® V2
Materials
related product
(±)-Amphetamine solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®S(+)-Amphetamine (dextro-Amphetamine) solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®R(-)-Amphetamine (levo-Amphetamine)
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®(±)-Methamphetamine solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®S(+)-Methamphetamine solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®R(-)-Methamphetamine solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Astec CHIROBIOTIC V2, 25 cm x 4.6 mm I.D., 5 μm (15024AST)
mobile phase
[A] methanol; [B] water; [C] acetic acid; [D] ammonium hydroxide; (95:5:0.1:0.02, A:B:C:D)
flow rate
1 mL/min
pressure
840 psi (58 bar)
column temp.
40 °C
detector
UV, 205 nm
injection
5 μL
sample
100 μg/mL each enantiomer in methanol
Description
Analysis Note
Resolution of the enantiomers of methamphetamine and amphetamine is of clinical interest for two reasons: they exhibit different physiological effects, and it is a means to distinguish between legal and illicit sources of the drugs. Shown here is the rapid and efficient resolution of all four enantiomers on an Astec CHIROBIOTIC V2 column. LC/MS grade solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC