HPLC Analysis of Erectile Dysfunction Drugs on Ascentis® Express RP-Amide
Materials
related product
Product No.
Description
Pricing
Sildenafil solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Tadalafil solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Vardenafil dihydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express RP-Amide, 10 cm x 3.0 mm I.D., 5 μm particles (50753-U)
mobile phase
[A] water with 0.1% formic acid; [B] acetonitrile with 0.1% formic acid;
gradient
5% B held for 0.5 min; to 100% B in 9.5 min; 100% B held for 2 min
flow rate
0.4 mL/min
pressure
870 psi (60 bar)
column temp.
35 °C
detector
UV, 250 nm
injection
2 μL
sample
20 μg/mL in water:methanol (85:15)
Description
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany