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HPLC Analysis of Four Low Moleculer Weight Proteins on 5μm BIOshell™ 160Å Peptide C18 Column, Comparison to Totally Porous 3 μm C18 Column

HPLC Analysis of Four Low Moleculer Weight Proteins on 5μm BIOshell™ 160Å Peptide C18 Column, Comparison to Totally Porous 3 μm C18 Column application for HPLC

Materials

related product

Product No.
Description
Pricing

L6876

Lysozyme from chicken egg white

powder or granules, ≥39,000 units/mg protein

R6513

Ribonuclease A from bovine pancreas

Molecular Biology, ≥70 Kunitz units/mg protein, lyophilized

L6010

α-Lactalbumin from bovine milk

Type III, calcium depleted, ≥85% (PAGE), lyophilized powder

C6749

Cytochrome c equine

recombinant, expressed in E. coli

67015-U

BIOshell™ A160 Peptide C18, 5 μm HPLC Column

5.0 μm particle size, L × I.D. 15 cm × 4.6 mm

CONDITIONS

column

BIOshell A160 Pejptide C18, 15 cm x 4.6 mm, 5 μm particles (67015-U)

mobile phase

[A] Water with 0.1% TFA, [B] Acetonitrile with 0.1% TFA

gradient

28-55% B in 5 min

flow rate

1.5 mL/min

pressure

1378 psi BIOshell (95 bar)

pressure

2465 psi Totally Porous (170 bar)

column temp.

60 °C

detector

UV, 280 nm

injection

15 μL

sample

Water with 0.1% TFA

Description

Analysis Note

The 5μm BIOshell 160Å Peptide C18 column offers increased resolution, faster separations, and lower back pressure when compared to a totally porous 3μm C18 column. In addition, the BIOshell column offers a level of ruggedness that is not often associated with columns exhibiting such a high level of performance.

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

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