CN
HPLC Analysis of Fusarium Mycotoxins on Ascentis® Express Phenyl-Hexyl
Materials
analytical column
Product No.
Description
Pricing
Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column
2.7 μm particle size, L × I.D. 10 cm × 2.1 mmCONDITIONS
column
Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)
mobile phase
[A] water, acetonitrile, 90:10; [B] acetonitrile
gradient
0% B held for 0.5 min; to 15% B in 1 min; held at 15% B for 6 min
flow rate
0.3 mL/min
pressure
192 bar (2780 psi)
column temp.
30 °C
detector
UV, 218 nm
injection
10 μL
sample
5 mg/L ea. in 95:5, water:acetonitrile
Description
Analysis Note
The trichothecene vomitoxins of Fusarium are significant plant pathogens of grain and forage crops that can lead to anorexic effects with livestock. This application demonstrates resolution of deoxynivalenol and three of its analogs.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany