HPLC Analysis of Protein Standard on BIOshell A400 Protein C18

column

BIOShell A400 C18, 10 cm x 2.1 mm I.D., 3.4 μm particles (67463-U)

mobile phase

[A] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 75:25; [B] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 25:75

gradient

0 to 75% B in 15 min

flow rate

0.3 mL/min

pressure

2800 psi (193 bar)

column temp.

30 °C

detector

UV, 215 nm

injection

2 μL

sample

ribonuclease A, cytochrome c, holo-transferrin and myoglobin 1 mg in mobile phase A;