HPLC Analysis of Proteins on BIOshell A400 Protein C4, Rapid Separation at Elevated Temperature
Materials
related product
Product No.
Description
Pricing
L-Lactic Dehydrogenase from rabbit muscle
Type XI, lyophilized powder, 600-1,200 units/mg proteinCONDITIONS
column
BIOshell A400 Protein C4, 5 cm x 2.1 mm I.D., 3.4 μm particles (66824-U)
mobile phase
[A] 75:25 (0.1% TFA in water):(0.1% TFA in acetonitrile); [B] 25:75 (0.1% TFA in water):(0.1% TFA in acetonitrile)
gradient
12 to 100% B in 1 min; held at 100% B for 1 min
flow rate
0.4 mL/min
pressure
905 psi (62 bar)
column temp.
90 °C
detector
UV, 215 nm
injection
1 μL
sample
protein mix in 0.05% TFA
Description
Analysis Note
This application demonstrates the suitability of BIOshell A400 Protein C4 column for fast elution of proteins via ballistic gradients. High temperature enhances chromatographic performance and lowers operational backpressure. The highest grade solvents provided clean, robust operation.
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