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HPLC Analysis of Proteins with Different pI Values on Proteomix® SCX-NP3

HPLC Analysis of Proteins with Different pI Values on Proteomix® SCX-NP3 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Z777151

Proteomix® SCX-NP3 Strong Cation Exchange Column

L × I.D. 5 cm × 4.6 mm, 3 μm particle size, stainless steel

standard

Product No.
Description
Pricing

A2512

Albumin from chicken egg white

lyophilized powder, ≥98% (agarose gel electrophoresis)

L6876

Lysozyme from chicken egg white

lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

A1153

Aprotinin from bovine lung

lyophilized powder, 3-8 TIU/mg solid

R6513

Ribonuclease A from bovine pancreas

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

CONDITIONS

column

Proteomix SCX-NP3, 5 cm x 4.6 mm I.D., 3 μm particles (Z777151)

mobile phase

(A) 10 mM sodium phosphate, pH 6.0; (B) A + 1 M NaCl

gradient

0 to 100% B in 15 min

flow rate

0.5 mL/min

detector

UV, 280 nm

Description

Legal Information

Proteomix is a registered trademark of Sepax Technologies

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Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS