HPLC Analysis of Sulfa Drugs on Titan C18

column

Titan C18 5 cm x 2.1 mm I.D., 1.9 μm particle (577122-U)

mobile phase

[A] water with 0.1% acetic acid; [B] acetonitrile with 0.1% acetic acid ; (90:10, A:B)

flow rate

0.4 mL/min

pressure

2670 psi (184 bar)

column temp.

40 °C

detector

UV, 250 nm

injection

2 μL

sample

25 μg/mL in 70:30, water:methanol