HPLC Analysis of Tetramisole Enantiomers and Related Compounds on Astec® CYCLOBOND™ I 2000 DMP
Materials
analytical column
standard
CONDITIONS
column
Astec CYCLOBOND I 2000 DMP 10 cm X 2.1 mm
mobile phase
(A) 100 mM ammonium acetate, pH 5 with acetic acid (B) acetonitrile, 90:10, v/v
flow rate
0.2 mL/min
pressure
360 psi
column temp.
35 °C
detector
UV, 220 nm
injection
2 μL
sample
100 ug/mL tetramisole and 50 ug/mL each cocaine and aminorex in water:methanol, 80:20, v/v
Description
Analysis Note
Cocaine is often found laced with levamisole (one of the tetramisole enantiomers) and aminorex is a metabolite of levamisole known to cause serious vascular damage in drug abusers. These compounds are readily separated using the Astec CYCLOBOND column - a derivatized cyclodextrin column only offered by Supelco. What is really interesting about this separation is that it shows the ability of chiral HPLC columns to separate enantiomers (here, dexamisole and levamisole) by chiral discrimination, as well as non-chiral discrimination in the same analysis. The compounds aminorex and cocaine are not enantiomers of each other or of tetramisole. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CYCLOBOND is a trademark of Sigma-Aldrich Co. LLC