HPLC-CAD Analysis of Lipids (Four) in a Lipid Nanoparticle (LNP) Formulation Containing mRNA
Materials
related product
CONDITIONS
column
Ascentis® C18, 15.0 cm x 2.1 mm, 3.0 µm (581302-U)
mobile phase
[A] Ammonium acetate in water (20 mM); [B] Ammonium acetate in methanol (20 mM)
flow rate
0.5 mL/min
column temp.
55 °C
detector
CAD (evaporation temp: 30 °C; data collection rate: 10 Hz, Filter: 5.0, Peak Width: 0.02 min)
injection
10 µL
sample/matrix
Undiluted LNP formulation encapsulating mRNA (0.1 mg/mL)
Description
General description
Lipid nano particles (LNP) are used as protective capsules to deliver nucleic acid to target cells, e.g. In COVID-19 vaccines, an mRNA was encapsulated.
Analysis Note
For in-production and final quality control, determination of LNP content is of interest. Here four lipids were determined by HPLC-CAD (Charged Areosol Detector) in an undiluted LNP formulation encapsulating an mRNA (0.1 mg/mL):
Other Notes
APP_718
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany