UHPLC Analysis of Corticosteroids on Titan C18
CONDITIONS
column
Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)
mobile phase
[A] water; [B] acetonitrile; (70:30, A:B)
gradient
30% to 60% B in 3 minutes, hold for 0.2 min
flow rate
0.4 mL/min
pressure
7423 psi (512 bar)
column temp.
35 °C
detector
UV, 240 nm
injection
1 μL
sample
1000 ng/ml in mobile phase
detector
ESI (+) MS TIC SIR
Description
Analysis Note
Analysis of Corticosteroids on a Titan C18 UPLC column with MS detection in ESI (+) Mode
Other Notes
Other MS Conditions:
Legal Information
null