US EPA Method 8315 (Solid Waste Carbonyls by HPLC-UV) on Ascentis® RP Amide
Materials
accessory
Product No.
Description
Pricing
Wheaton wide-mouth bottle
amber soda-lime glass bottle, capacity 1000 mL, white polypropylene cap, (PTFE faced LDPE liner), case of 12 eaCoupler for Legacy Guard Column Holder
PEEK, I.D. × O.D. × Overall L 0.010 in. × 1/16 in. × 1 in.Supelguard Guard Cartridge Holder
Stand-alone, for use with Supelguard cartridges (2 cm L. x 2.1 to 4.0 mm I.D.)related product
Product No.
Description
Pricing
Ascentis® RP-Amide Supelguard Guard Cartridge
5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 eaused together
Product No.
Description
Pricing
Ascentis® C18 Supelguard Guard Cartridge
5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 eaAscentis® C18 Supelguard Guard Cartridge
3 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 eaAscentis® C8 Supelguard Guard Cartridge
5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 eaCONDITIONS
column
Ascentis RP-Amide, 25 cm x 4.6 mm I.D.,5 μm particles (565325-U)
mobile phase
A [A] DI water; [B] acetonitrile; (60:40, A:B)
gradient
0 min 75% A; 15 min 5% A; 0 min 25% B; 15 min 95% B; A:B held for 5 min
flow rate
1.5 mL/min
column temp.
40 °C
detector
UV, 360 nm
injection
10 μL
sample
(as listed in mobile phase)
mobile phase
B [A] DI water; [B] acetonitrile; (25:75, A:B)
Description
Analysis Note
This application demonstrates the suitability of the Ascentis RP-Amide for the analysis of 21 aldehyde/ketone DNPH derivatives.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany