Ascentis RP-Amide, 25 cm x 4.6 mm I.D.,5 μm particles (565325-U)
A [A] DI water; [B] acetonitrile; (60:40, A:B)
0 min 75% A; 15 min 5% A; 0 min 25% B; 15 min 95% B; A:B held for 5 min
1.5 mL/min
40 °C
UV, 360 nm
10 μL
(as listed in mobile phase)
B [A] DI water; [B] acetonitrile; (25:75, A:B)
This application demonstrates the suitability of the Ascentis RP-Amide for the analysis of 21 aldehyde/ketone DNPH derivatives.
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany