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US EPA Method 8315 (Solid Waste/Ground Water Carbonyls by HPLC-UV) on Ascentis® C18

US EPA Method 8315 (Solid Waste/Ground Water Carbonyls by HPLC-UV) on Ascentis® C18 application for HPLC

Materials

accessory

Product No.
Description
Pricing

Wheaton wide-mouth bottle

amber soda-lime glass bottle, capacity 1000 mL, white polypropylene cap, (PTFE faced LDPE liner), case of 12 ea

Coupler for Legacy Guard Column Holder

PEEK, I.D. × O.D. × Overall L 0.010 in. × 1/16 in. × 1 in.

Supelguard Guard Cartridge Holder

Stand-alone, for use with Supelguard cartridges (2 cm L. x 2.1 to 4.0 mm I.D.)

related product

Product No.
Description
Pricing

Ascentis® C18 HPLC Column

3 μm particle size, L × I.D. 15 cm × 4.6 mm

Ascentis® C18 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

Ascentis® RP-Amide Supelguard Guard Cartridge

5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea

Ascentis® C8 HPLC Column

3 μm particle size, L × I.D. 15 cm × 4.6 mm

Ascentis® C8 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

Ascentis® Express C18 (2.7 μm) HPLC Column

L × I.D. 15 cm × 4.6 mm, pk of 1 ea

Ascentis® Express C8, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

used together

Product No.
Description
Pricing

Ascentis® C18 Supelguard Guard Cartridge

5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea

Ascentis® RP-Amide HPLC Column

3 μm particle size, L × I.D. 15 cm × 4.6 mm

Ascentis® RP-Amide HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

Ascentis® C18 Supelguard Guard Cartridge

3 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea

Ascentis® C8 Supelguard Guard Cartridge

5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea

CONDITIONS

column

Ascentis C18, 15 cm x 4.6 mm I.D., 3 μm particles (581322-U)

mobile phase

30:70 water:acetonitrile

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 365 nm

injection

1 μL

sample

15-component DNPH-derivatized carbonyl mix, each analyte at
15 μg/mL (aldehyde equivalent) in acetonitrile (47285-U)

Description

Analysis Note

This application demonstrates the suitability of the Ascentis C18 for the analysis of 15 aldehyde/ketone DNPH derivatives.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany