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LC/MS Analysis of Anti-infectives on Ascentis® Express Biphenyl

LC/MS Analysis of Anti-infectives on Ascentis® Express Biphenyl application for LC-MS

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express Biphenyl Column

2.7 μm particle size, L × I.D. 10 cm × 3.0 mm

mobile phase component

Product No.
Description
Pricing

Ammonium formate

eluent additive for LC-MS, LiChropur, ≥99.0%

standard

Product No.
Description
Pricing

Cinoxacin

p-Coumaric acid

≥98.0% (HPLC)

Dapsone

VETRANAL®, analytical standard

CONDITIONS

column

Ascentis Express Biphenyl, 10 cm x 3.0 mm, 2.7 μm particles (64066-U)

gradient

10 mM ammonium formate (70:30 water:acetonitrile) SW pH 4.00

flow rate

0.6 mL/min

pressure

997 psi (68.7 bar)

column temp.

35 °C

detector

MS, ESI+, XIC

injection

5 μL

sample

300ng/mL in 85:15,  water:methanol

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany