mPAGE® Lux Casting System for SDS-PAGE Gels
Discover the mPAGE® Lux Casting System for ready-to-use SDS-PAGE gels in 3 minutes. Fresh SDS-PAGE gels that are ready when you need them.
mPAGE® Lux Casting System for SDS-PAGE Gels
How do you cast a gel for SDS-PAGE? Gel casting typically involves pouring resolving and stacking gels in a time-consuming, stepwise process. Researchers traditionally have few options to meet their gel casting and electrophoresis needs. Our innovative mPAGE® Lux SDS-PAGE Gel Casting System is designed to provide the flexibility of hand-cast gels and the reliability of precast gels, without the loss of quality, time, or the significantly higher cost that accompany the alternative options. Gels in less than three minutes with our mPAGE® Lux Casting System. You can say farewell to conventional methods and their time consuming, manual steps for gel creation. That’s fresh.
mPAGE® Lux Casting System Features and Benefits
mPAGE® Lux Casting System replaces your gel casting process with faster, simpler, and safer methods with more reproducible results. Cure a mini gel in 90 seconds with the mPAGE® Lux Casting System compared to conventional methods that take more than 90 minutes. Our quick casting workflow with UV irradiation expedites curing of mPAGE® Lux Bis-Tris reagents while Bis-Tris gel chemistry allows for shorter run times compared to conventional methods.
The one-step photopolymerization using precise system controls along with less preparation steps means there are fewer chances for error. Our mPAGE® Caster prevents leakage and the use of Bis-Tris gels provides superior band quality compared to hand-cast Tris-Glycine gels. Additionally, the mPAGE® Lux Reagent Kit, mPAGE® TurboMix Reagent Kit, and mPAGE® precast gels all use Bis-Tris chemistry and share the same running buffers and gel stains. Our mPAGE® Lux Casting System does not use APS or TEMED and offers a safer option using fewer toxic chemicals.
Traditional Hand Cast Workflow
mPAGE®Lux Workflow
Figure 1. Traditional Gel Casting vs the mPAGE® Lux Workflow. The mPAGE® Lux Casting system has fewer overall steps, reduced polymerization time, and toxic waste compared to traditional gel casting technology. Gels created by mPAGE® Lux Casting System are compatible with mPAGE® Mini-gel Tank and the Bio-Rad Mini-PROTEAN Cells. For outstanding resolution and reduced run times, use our mPAGE® buffers. Lux gels are compatible with Bis-Tris compatible buffers and staining reagents.
What are the steps of gel casting?
Casting an SDS-PAGE gel often includes weighing, premixing gel solutions, casting, and wait time for polymerization. While often cited as a more economical solution, conventional hand-cast gel methods are time consuming, requiring many hands-on steps which may result in inconsistent gel formulations. The quality of hand-cast gels and the data they may help generate rely on formulation consistency so that precious reagents and time are ultimately not wasted with gel failures.
Figure 2.Data quality comparison with mPAGE® Lux Bis-Tris gel and hand-cast tris-glycine gel. Band quality of mPAGE® Lux Bis-Tris gel (left) compared to hand-cast tris-glycine gel (right). Both gels are 10% acrylamide and loaded with a titration of A431 human cell lysate. Electrophoresis run time is 42 minutes at 200V (Lux gel) and 119 minutes at 120V (tris-glycine gel). Gels are stained with ReadyBlue™ Coomassie gel stain.
The mPAGE® Lux Casting System offers an innovative solution to your gel casting process. You no longer must choose either saving time or resources. Our novel gel casting system allows researchers to easily cast their gels quickly when they need them, at a fraction of the cost of precast gel technologies. Improve your lab efficiency and upgrade your gel electrophoresis workflow with the mPAGE® Lux Casting System.
Figure 3. Western blotting for wet, fast, and semi-dry transfer method comparison. Comparisons were performed using 12% mPAGE® Lux Bis-Tris gels that were run with a titration of A431 human cell lysate and with various transfer methods onto Immobilon®-P membranes. Membranes were blotted with anti-EGFR and anti-ErK1/2 antibodies, then detected with Immobilon® ECL Ultra Western HRP substrate. Turbo method = transfer using Bio-Rad Trans-Blot® Turbo™ Transfer System and Trans-Blot® Turbo™ Transfer Packs.
Figure 4: Reproducibility of mPAGE® Lux Bis-Tris gels. A. Four 8% gels were cast and run with titration of A341 human cell lysate subsequently stained with ReadyBlue™ Coomassie gel stain. B. The relative migration distance (Rf) for the unstained molecular weight marker was calculated for each gel. Standard deviation is shown for each protein band.
Figure 5.mPAGE® Lux Bis-Tris reagent shelf-life analysis. mPAGE® Lux Bis-Tris gels cast using reagents (A) 0 months, (B) 6 months, or (C) 12 months old. No difference is observed in running pattern or run time.
mPAGE® Lux Casting System and Bis-Tris Reagent Kits
Add mPAGE® Lux Casting System to your lab and have ready-to-use gels in under 3 minutes. Take a breath and get used to a fast gel casting process with less waste and without the worry of failure. That’s fresh. Our mPAGE® Lux Casting System Kits consist of the mPAGE® Lux Curing Station, mPAGE® Lux Mixing Tube, mPAGE® Gel Caster, mPAGE® Comb with 10 wells and 15 wells, mPAGE® Mini Spacer Plate, mPAGE® Lux Masked Short Plates with 10 wells and 15 wells. For the 0.75 mm thickness mPAGE® Lux Casting system, the 15 well comb and masked short plates are not included. Kit descriptions and configurations follow:
mPAGE® Lux Curing Station
The mPAGE® Lux Bis-Tris Reagent Kit is a dedicated reagent kit for the mPAGE® Lux Casting System. The mPAGE® Lux Bis-Tris Reagent Kit consists of 3 components, Resolving Solution, Diluent, and Stacking Solution. The kit supports a variety of acrylamide gels between 8.0-13.5%. mPAGE® Lux Reagent Kit description and configuration follows:
mPAGE® Lux SDS-PAGE Gel Cast Protocol
All solutions must be protected from sunlight to prevent polymerization. Before casting gels, bring all solutions to room temperature.
Resolving Gel
- Prepare Resolving Gel solution by mixing Resolving Solution and Diluent. See the table below for mixing volumes. Resolving gel solution may be prepared in a large batch to make several gels sequentially.
- Using a clean pipette, add Resolving Solution to a black mixing tube or other opaque container.
- Using a clean pipette, add Diluent to the same mixing tube.
- Mix by gently turning container end over end. Do not vortex.
Resolving Gel Solution Volumes
Use the below volumes to make a single 1.0 mm gel. For 0.75 mm and 1.5 mm solution volumes, see the full user guide available on the product page at SigmaAldrich.com.
Stacking Gel
The Stacking Solution is ready to use directly from the bottle. Use 1.5 mL Stacking Solution for a 1.0 mm gel. Important: Do not dilute Stacking Solution.
Gel Casting
- Clean glass plates with mild detergent and rinse with DI water. Wipe with 70% Ethanol before use.
- Assemble mPAGE® Gel Caster using mPAGE® Spacer Plate and mPAGE® Lux Masked Short Plate to form glass cassette. The masked short plate should be oriented with the text on the left side of the cassette. Ensure that both glass plates are aligned at the bottom of the caster frame before closing caster clamps. Note: The mPAGE® Lux Masked Short Plate must be used with the mPAGE® Curing Station.
- Power on mPAGE® Lux Curing Station by pressing the power button. The Curing Station will initiate a self-test. After self-test is complete, the ready screen will appear.
- Open door and place gel caster into Curing Station, aligning caster behind the bumpers.
- Using a clean 5 mL pipette, add prepared Resolving Gel Solution to the indicated fill line on the mPAGE® Gel Caster frame.
- Using a clean 5 mL pipette, slowly add Stacking Solution up to the top of the short plate.
- Slowly insert the mPAGE® comb at an angle to prevent air bubble formation under teeth. Important: Mixing different well formats may result in improper well formation. Use:
- 10-well combs with 10-well mPAGE® Lux Masked Short Plates
- 15-well combs with 15-well mPAGE® Lux Masked Short Plates
- Wipe solution spill-over from front of short plate to prevent incomplete curing where spill-over exists.
- Close Curing Station door and select gel thickness.
- Press Start to begin gel curing. Tip: If casting multiple gels, begin the Gel Casting process with second Gel Caster now, while the first gel is curing.
- After curing is complete, open door and remove Gel Caster. Remove cassette from Gel Caster by lowering the tension clip to release the caster frame. Then open the sides of the frame and slide cassette out from top.
- Use gel immediately or wrap the cassette in wet paper towel and store lying flat in a zip top bag or other airtight container at 2-8 °C for up to 2 weeks. Do not leave cassettes unwrapped as the gel will dry out.
How To Remove Gel After Electrophoresis
Remove the gel from glass cassette using a gel scraper. Cut along gel edges (right) to avoid gel tearing. mPAGE® Lux Bis-Tris gels should ONLY be used with MOPS-SDS or MES-SDS running buffer. Bis-Tris gels are NOT compatible with Tris-Glycine running buffer.
Sustainable gel casting with mPAGE® Lux Gel Casting System
We are committed to delivering greener workflow solutions that enable our customers to complete their protein resolution research while reducing waste and chemical hazards. Our mPAGE® Lux Gel Casting System reduces the amount of single-use plastic waste generated per gel by up to 26% and reduces the time and number of steps necessary to create a gel compared to traditional hand cast workflows. The mPAGE® Lux System also uses photo-initiators to replace hazardous reagents, including TEMED and APS, eliminating exposure to these reagents and the need to manage associated wastes. Discover the impactful time saving and environmental benefits of our mPAGE® Lux System as we work together towards a more sustainable future.
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