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MISSION® Predesigned siRNA were created using the proprietary Rosetta Inpharmatics siRNA Design algorithm in an exclusive partnership with Merck & Co. The Rosetta siRNA Design Algorithm utilizes Position-Specific Scoring Matrices and knowledge of the seed region to predict the most specific and effective sequences for your target genes. The algorithm’s rules were developed utilizing empirical data collected from gene silencing experiments carried out over three years.

Product Benefits

  • Best-in-class, guaranteed gene silencing
  • Efficient knockdown of low abundance messages
  • Simplified transfection optimization with 11 Positive Control siRNA
  • Distinguish sequence-specific silencing from non–specific effects with 8 negative control siRNA
  • Hundreds of functionally validated predesigned siRNA

If you have needs that are different from the general specifications presented here, please send a request to sirnarequest@milliporesigma.com.

Product Pools

A popular pooled format is 4 duplexes at 5 nmol each combined into one tube (20 nmol pooled) plus the exact same 4 duplexes also at 5 nmol each in separate tubes (another 20 nmol individual). However, our sophisticated liquid handlers allow for a wide range of other possibilities. For feasibility review of your specific needs, please email sirnarequest@milliporesigma.com.

Validated Predesigned siRNA

Many common gene targets have been validated for ≥75% mRNA knockdown (Figure 1 shows example data and the table for a list of commonly-ordered, validated siRNA by gene symbol). Validated siRNA are suitable for transfection optimization and as positive controls.

HeLa cells transfected with predesigned siRNA at a concentration of 30 nM

Figure 1.HeLa cells transfected with predesigned siRNA at a concentration of 30 nM

Predesigned Positive Controls

MISSION® Positive Control siRNA serve as an ideal complement to any gene silencing experiment. Positive controls are validated siRNA known to achieve high levels (>70%) of knockdown. A positive control should be used to optimize transfection, and if it fails to produce the expected phenotype, adjustments to experimental conditions are likely necessary.

Available Positive Controls by Gene Target:

GAPDH, MAPK1, KIF11, LMNA, PPIB, TP53

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany and/or its affiliates. Label License.

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