Research area: Cell Signaling

Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidasecitation and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.

Optimal pH: 3.5-6.0.

Cathepsin B, Human Liver has been used to assess its linker cleavage or proteolytic activity using an LC/MS MRM-based quantitation method.

Cathepsin B facilitates non-specific, bulk proteolysis within the acidic conditions of the endosomal/lysosomal compartment. Here, they break down both intracellular and extracellular proteins. Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.

Please refer to vial label for lot-specific concentration.

In 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.

Following initial thaw, aliquot and freeze (-70°C).

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Kostoulas, G., et al. 1999. FEBS Lett. 455, 286. Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281. Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117. Musil, D., et al. 1991. EMBO J.10, 2321. Sloane B.F. 1990. Semin. Cancer Biol.1, 137.

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-βNA per min at 40°C, pH 6.0 in the presence of DTT.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)