219364-M
Cathepsin B, Human Liver
Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.
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Specific activity:
≥50 units/mg protein
Assay:
>95% (SDS-PAGE and HPLC)
Biological source:
human liver
biological source
human liver
description
Merck USA index - 14, 1905
assay
>95% (SDS-PAGE and HPLC)
form
liquid
specific activity
≥50 units/mg protein
purified by
affinity chromatography
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
technique(s)
activity assay: suitable
pH range
3.5-6.0
suitability
suitable for molecular biology
application(s)
life science and biopharma
shipped in
wet ice
storage temp.
−70°C
Quality Level
Gene Information
human ... CTSB(1508)
General description
Research area: Cell Signaling
Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidasecitation and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.
Optimal pH: 3.5-6.0.
Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidasecitation and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.
Optimal pH: 3.5-6.0.
Application
Cathepsin B, Human Liver has been used to assess its linker cleavage or proteolytic activity using an LC/MS MRM-based quantitation method.
Biochem/physiol Actions
Cathepsin B facilitates non-specific, bulk proteolysis within the acidic conditions of the endosomal/lysosomal compartment. Here, they break down both intracellular and extracellular proteins. Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Other Notes
Kostoulas, G., et al. 1999. FEBS Lett. 455, 286. Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281. Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117. Musil, D., et al. 1991. EMBO J.10, 2321. Sloane B.F. 1990. Semin. Cancer Biol.1, 137.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-βNA per min at 40°C, pH 6.0 in the presence of DTT.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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