234154
Human Collagen Type IV
from human placenta, liquid, suitable for cell culture
别名:
Collagen, Type IV, Human Placenta, Native, type IV collagen
产品名称
Collagen, Type IV, Human Placenta, (100 μg), solution, suitable for cell culture, purified by (Highly purified), Native protein
biological source
human placenta
description
Merck USA index - 14, 2480
assay
≥90% (SDS-PAGE)
form
solution
purified by
(Highly purified)
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
concentration
(100 μg)
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
storage temp.
−20°C
Quality Level
Application
The human placental collagen type IV (Sigma Aldrich 234154) is used:
- as a coating substrate for cell culture treated flasks to promote the growth and adhesion of CuFi-1 cells, providing reliable conditions for cell line propagation and maintenance
- to coat transwell inserts, providing a suitable substrate for the growth and formation of monolayers in intestinal organoid culture for trans epithelial electrical resistance (TEER) measurement and drug treatments
- to coat inserts for two-dimensional monolayer culture
Biochem/physiol Actions
Collagen Type IV interacts with numerous binding partners. Type IV collagen forms a structural scaffold within the basement membrane (BM) along with other macromolecules like laminins, heparan sulfate proteoglycans, fibronectin, and entactin. This complex network regulates the interaction between the BM and adhering cells, providing critical support and functionality. Thereby, regulates cell adhesion and migration.
Disclaimer
Toxicity: Standard Handling (A)
General description
Collagen Type IV is a primary component of the basement membrane (BM). It is a specialized sheet-like extracellular matrix that surrounds specific cell types such as skeletal muscle cells, smooth muscle cells, heart muscle cells, and adipocytes in multicellular tissues. Collagen type IV is characterized as a nonfibril-forming collagen and is composed of six genetically distinct α-chain polypeptides. These chains have the potential to form 56 different combinations, resulting in various homotrimers. Through self-assembly, collagen type IV constructs organized networks that play a crucial role in maintaining the stability and assembly of the basement membrane (BM). Its presence is essential for the structural integrity and functionality of the BM.
Other Notes
Miller, E.J., and Gay, S. 1987. Methods Enzymol. 144, 3.
Klasson, S.C., et al. 1986. Coll. Relat. Res.6, 397.
Glanville, R.W., et al. 1979. Eur. J. Biochem.95, 383.
Klasson, S.C., et al. 1986. Coll. Relat. Res.6, 397.
Glanville, R.W., et al. 1979. Eur. J. Biochem.95, 383.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In sterile 2 mM HCl.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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