form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
shipped in
wet ice
General description
A single component reagent for chemiluminescent detection of immobilized proteins, labeled with horseradish peroxidase, by immunoblotting. Provides a bright, stable signal with low signal:background. Does not require mixing of substrate and enhancer solutions. Volume is sufficient for covering up to 2000 cm2 of membrane.
A single component reagent for chemiluminescent detection of immobilized proteins, labeled with horseradish peroxidase, by immunoblotting. Volume supplied is sufficient for covering up to 2000 cm2 of membrane.
RapidStep ECL is a 1 STEP chemiluminescent substrate reagent for detection of horseradish peroxidase (HRP) on immunoblots. It is a convenient, cost-effective, and ready-to-use reagent that provides a superior, longer-lasting signal than traditional ECL reagents.
RapidStep contains luminol and enhancer combined in one solution and is ready-to-use without mixing. A spray bottle is used to apply the solution directly onto the membrane.
RapidStep contains luminol and enhancer combined in one solution and is ready-to-use without mixing. A spray bottle is used to apply the solution directly onto the membrane.
This RapidStep ECL Reagent is validated for use in Immunoblotting for the detection of RapidStep ECL Reagent.
Application
Immunoblotting
Other Notes
To achieve an optimal signal:noise ratio, it is necessary to optimize the concentration of primary and secondary antibodies.
Protocol for Use
1. Remove the membrane containing immobilized proteins from the last wash and place on a flat surface, protein side facing up. Do not allow the membrane to dry
2. Spray RapidStep ECL Reagent on the surface of the membrane to sufficiently cover the entire membrane. Gently shake the container to ensure the membrane is evenly covered. Generally 2 sprays are sufficient to cover a 5 cm x 5 cm area.
3. Incubate the membrane for 1 min at room temperature without agitation. Proteins with low specificity or low reactivity with the primary antibody may require longer incubation times.
4. Drain the excess RapidStep ECL Reagent by holding the membrane vertically and touch the edge of the membrane against absorbent paper. Failure to drain sufficiently may lead to high background.
5. Wrap the membrane in plastic wrap and gently smooth out air pockets.
6. Place the membrane in a film cassette, protein side facing up.
7. Place X-ray film on top of the membrane, close the cassette and expose for an appropriate period of time. Optimal exposure times should be determined for each experiment.
Protocol for Use
1. Remove the membrane containing immobilized proteins from the last wash and place on a flat surface, protein side facing up. Do not allow the membrane to dry
2. Spray RapidStep ECL Reagent on the surface of the membrane to sufficiently cover the entire membrane. Gently shake the container to ensure the membrane is evenly covered. Generally 2 sprays are sufficient to cover a 5 cm x 5 cm area.
3. Incubate the membrane for 1 min at room temperature without agitation. Proteins with low specificity or low reactivity with the primary antibody may require longer incubation times.
4. Drain the excess RapidStep ECL Reagent by holding the membrane vertically and touch the edge of the membrane against absorbent paper. Failure to drain sufficiently may lead to high background.
5. Wrap the membrane in plastic wrap and gently smooth out air pockets.
6. Place the membrane in a film cassette, protein side facing up.
7. Place X-ray film on top of the membrane, close the cassette and expose for an appropriate period of time. Optimal exposure times should be determined for each experiment.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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