Milli-Mark^® Anti-Ubiquitin Antibody, Lys48-Specific-FITC, clone Apu2

Molecular weight varies

Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.

Epitope: Lys48 ubiquitination site

Immunogen used was di-ubiquitin linked at Lys48

Milli-Mark Anti-Ubiquitin Antibody, Lys48-Specific-FITC, clone Apu2 is an antibody against Ubiquitin for use in FC.

Research Category
Signaling

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Recognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin.

Protein A purified

Purified recombinant rabbit monoclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.

Control
HeLa cells

Evaluated by flow cytometry using HeLa cells

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