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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
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生物来源

rabbit

质量水平

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

种属反应性

rat

种属反应性(根据同源性预测)

bovine

技术

immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

NCBI登记号

UniProt登记号

运输

ambient

靶向翻译后修饰

unmodified

基因信息

human ... CLIC4(25932)

一般描述

Chloride intracellular channel protein 4 (UniProt: Q9XSA7; also known as Intracellular chloride ion channel protein p64H1, CLIC4) is encoded by the CLIC4 gene (Gene ID: 286823) in bovine. CLIC4, a member of the CLIC family of proteins. CLIC family members can change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion. CLIC4 is ubiquitously expressed and is present in both soluble and membrane-associated forms. It is present in the cytosol as a diffusible protein, but is reported in endoplasmic reticulum, trans-Golgi network, mitochondria, caveolae, dense-core secretory vesicles in the central nervous system, and in nuclei of differentiating keratinocytes. CLIC4 is rapidly recruited to the plasma membrane by lysophosphatidic acid (LPA) and serum, indicating its possible role in exocytic endocytic trafficking. CLIC4 also participates in such diverse processes as angiogenesis, differentiation, migration and wound healing. It is reported to stimulate both the internalization and serum/ LPA-induced recycling of beta1 integrin. In developing and developed kidneys, CLIC4 is enriched in the proximal tubule epithelial cells where it is considered to be essential for luminal delivery, microvillus morphogenesis, and endo-lysosomal biogenesis. CLIC4-null mice embryos exhibit impaired renal tubulogenesis and have smaller kidneys with fewer glomeruli and less dense peritubular capillary network. (Ref.: Edwards, JC et al. (2014). BMC Nephrology 15:54; Argenzio, A et al. (2014). J. Cell Sci.127 (24): 5189-5203).

特异性

This polyclonal antibody detects intracellular channel protein 4 in rat brain.

免疫原

Epitope: C-terminus
GST-tagged recombinant fragment corresponding to 158 amino acids from the internal region of bovine Chloride intracellular channel protein 4 (CLIC4).

应用

Detect Chloride intracellular channel protein 4 using this rabbit polyclonal Anti-CLIC4, Cat. No. ABS2088, used in Western Blotting and Immunofluorescence, Western Blotting, Immunohistochemistry (Paraffin) and Immunoprecipitation.
Immunoprecipitation Analysis: A representative lot detected CLIC4 in Madin-Darby canine kidney (MDCK) cells (Chou, S.Y., et. al. (2016). Nat Commun. 7:10412).

Immunohistochemistry Analysis: A representative lot detected CLIC4 in Immunohistochemistry applications (Chou, S.Y., et. al. (2016). Nat Commun. 7:10412).

Western Blotting Analysis: A representative lot detected CLIC4 in immunoblots of renal lysates harvested from WT and CLIC4-KO postnatal 0 (PN0 mice) (Chou, S.Y., et. al. (2016). Nat Commun. 7:10412).

Immunofluorescence Analysis: A representative lot detected CLIC4 in postnatal 0 (PN0) mouse renal paraffin and frozen sections (Chou, S.Y., et. al. (2016). Nat Commun. 7:10412).
Research Category
Signaling

质量

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1:500 dilution of this antibody detected CLIC4 in 10 µg of rat brain tissue lysate.

目标描述

~29 kDa observed; 28.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.02% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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