Hepatitis C virus (HCV) is a small (~55-65 nm), enveloped, positive-sense single-stranded RNA virus that is a causative factor for hepatitis C and hepatocellular carcinoma. HCV expresses several proteins that promote viral replication. Genome polyprotein of HCV can be cleaved into 11 different chains. The mature core protein packages viral RNA to form a viral nucleocapsid, and promotes virion budding. It also prevents the establishment of cellular antiviral state by blocking the interferon- a/b and interferon- g signaling pathways and by inducing STAT1 degradation. Envelope proteins E1 and E2 form a heterodimer, which is involved in virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. Viroprotein P7, a heptameric ion channel protein, plays an essential role in the assembly, envelopment, and secretion of viral particles. HCV polyprotein also contains NS2, a cysteine protease and NS3, a serine protease. NS2 is required for the proteolytic auto-cleavage between NS2 and NS3. NS3 binds a single ATP and catalyzes the unzipping of a single base pair of dsRNA. It also inhibits host antiviral proteins TBK1 and IRF3 and prevents the establishment of an antiviral state. Non-structural protein 4A (NS4A), a peptide cofactor, forms a non-covalent complex with the N-terminal of NS3 and this complex prevents phosphorylation of host IRF3, which also prevents the establishment of dsRNA induced antiviral state. NS4B induces a specific membrane alteration that serves as a scaffold for the virus replication complex. Non-structural protein 5A (NS5A), a multi-functional phosphoprotein, is an RNA-binding peripheral membrane protein that is reported to antagonize numerous cellular pathways, including the antiviral interferon- a response. It can bind to the 3′-ends of HCV plus and minus strand RNAs. NS5A exists as hypo- and hyper-phosphorylated forms and the dynamic transition between these two states is involved in the functions of NS5A. Hyperphosphorylation occurs primarily at its six serine residues within the low complexity sequence of NS5A. Phosphorylated NS5A is shown to be essential for viral replication and assembly. (Ref.: Kandangwa, M. and Liu, Q. (2019). Biochem. Biophys. Res. Commmun. 520(1); 192-197; Saeed, M., et al. (2015). Nature. 524(7566); 471-475; Huang, L., et al. (2005). J. Biol. Chem. 280(43); 36417-36428).

Purified Con1 HCV-non-structural 5A protein.

Quality Control Testing

Evaluated by Western blotting with recombinant Hepatitis C Virus NS5A protein.

Western Blotting Analysis (WB): A 1:100 dilution of this antibody detected recombinant Hepatitis C virus NS5A protein.

Tested Applications

Western Blotting Analysis: A representative lot detected HCV NS5A in Western Blotting applications (Lindenbach, B.D., et al. (2005). Science. 309(5734):623-6).

Immunocytochemistry Analysis: A representative lot detected HCV NS5A in Immunocytochemistry applications (Lindenbach, B.D., et al. (2005). Science. 309(5734):623-6).

Immunoprecipitation Analysis: A representative lot immunoprecipitated HCV NS5A in Immunopreciptation applications (Saeed, M., et al. (2015). Nature. 524(7566):471-5).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-HCV NS5A, clone 9E10, Cat. No. MABF2775, is a mouse monoclonal antibody that detects Hepatitis C virus NS5A protein and is tested for use in Immunocytochemistry, , Immunoprecipitation, and Western Blotting.

Clone 9E10 is a mouse monoclonal antibody that detects non-structural protein 5A (NS5A)of hepatitis C virus.

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.