The small intestine consists of continuous villi and crypts. The crypt is mainly a proliferative compartment and is maintained by multipotent stem cells whereas the villus represents the differentiated compartment, and it receives cells from multiple crypts. The functional compartment of the epithelium contains differentiated cells that populate the villi and can be categorized based on their function: enterocytes that function to absorb nutrients, goblet cells that secrete a protective mucus barrier, and enteroendocrine cells that release gastrointestinal hormones. Intestinal crypts contain intestinal stem cells (ISC), which are multipotent adult stem cells that reside in the base of crypts. They continuously self-renew by dividing and differentiate into the specialized cells of the intestinal epithelium, which renews throughout life. ISCs located either at the crypt base interspersed between the Paneth cells can be categorized into active-cycling (Lgr5+ cells) or those located near the þ4 position in the crypt as slow-cycling Bmi-1+ cells. Paneth cells that are responsible for secreting antibacterial peptides reside at the base of the proliferative compartment. As ISCs begin to differentiate, they migrate toward the lumen and are eventually shed, either from the tip of the intestinal villi or from the surface of the colonic epithelium. Clone F5C12 is suitable for detecting Lgr5+ cells and facilitates enrichment of Lg5GFP cells by FACS analysis. It does not detect Bmi-1+ or Lyz1 marker for Peneth cells. (Ref.: Smith, NR., et al. (2018). Cell. Mol. Gastroenterol. Hepatol. 6(1);79-96; Umar, S. (2010). Curr. Gastroenterol. Rep. 12(5); 340-348).

Clone F5C12 is a rat monoclonal antibody that detects Intestinal stem cells.

Undifferentiated Mouse intestinal epithelial cells. Rats were preimmunized with isolations of differentiated mouse intestinal epithelial cells and then treated with cyclophosphamide (i.p.) to eliminate B lymphocytes reacting against these antigens. Subsequent immunization was with crypt-based single cells.

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in mouse intestinal tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution of this antibody detected Intestinal stem cell in mouse intestinal tissue sections.

Tested Applications

Flow Cytometry Analysis: A representative lot detected Intestinal Stem Cell in Flow Cytometry applications (Smith, N.R., et al. (2018). Cell Mol Gastroenterol Hepatol. 6(1):79-96).

Immunohistochemistry Applications: A representative lot detected Intestinal Stem Cell in Immunohistochemistry applications (Smith, N.R., et al. (2018). Cell Mol Gastroenterol Hepatol. 6(1):79-96).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Intestinal Stem Cell, clone F5C12, Cat. No. MABS2231, is a rat monoclonal antibody that detects Intestinal stem cells and is tested for use in Flow Cytometry and Immunohistochemistry (Paraffin).

Purified rat monoclonal antibody IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.