The epithelium of the small intestine is shown to be organized into large numbers of self-renewing crypt-villus units. Crypts (of Lieberkuhn) are moat-like invaginations of the epithelium around the villi and are lined largely with young epithelial cells, which are involved primarily in secretion. At the base of crypts are stem cells that continually divide and are the source of active self-renewal of the epithelium in the crypt and villi. It has been shown that cells in the enterocyte lineage divide several times as they are pushed out of the crypts to actively migrate onto and along the villi. They differentiate further into the mature absorptive cells that express all the transport proteins and enzymes. Clone E5D10 is highly useful in identifying and isolating Lgr5+ and Bmi1+ intestinal stem cell populations. Although this antibody displays pan-epithelial staining pattern within the small intestine and down along the length of the intestine, it shows more robust staining on the surface of the differentiated epithelial cells on villi and the upper crypt within the small intestine. In the colon this antibody recognizes cells in the colonic cuff epithelium and approximately 2/3 of the way down the colonic crypt. (Ref.: Cleveres, H. (2013). Cell. 154(2); 274-284; Smith, NR., et al. (2018). Cell. Mol. Gastroent. Hepatol. 6(1); 79-96).

Clone E5D10 is a rat monoclonal antibody that detects differentiated epithelial cells on villi and the upper crypt within the small intestine and on the colonic crypt cuff epithelium.

Preimmunized with differentiated murine intestinal epithelial cells followed by cyclophosphamide (IP) to eliminate B cells and followed by immunization with crypt-based cells.

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in mouse intestinal tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution of this antibody detected Villus Crypt in mouse intestinal tissue sections.

Tested Applications

Immunohistochemistry Applications: A representative lot detected Villus Crypt in Immunohistochemistry applications (Smith, N.R., et. al. (2018). Cell Mol Gastroenterol Hepatol. 6(1):79-96).

Flow Cytometry Analysis: A representative lot detected Villus Crypt in Flow Cytometry applications (Smith, N.R., et. al. (2018). Cell Mol Gastroenterol Hepatol. 6(1):79-96).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Villus Crypt, clone E5D10, Cat. No. MABS2232, is a rat monoclonal antibody that detects Murine Villus Crypt and is tested for use in Flow Cytometry and Immunohistochemistry (Paraffin).

Purified rat monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at +2°C to +8°C from date of receipt.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.