NCI-H292
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91091815, human lung, Epithelial
别名:
H292 Cells, NCI-HUT-292 Cells
生物来源
human lung
包装
tube of 5 μg 91091815-DNA-5UG
pkg of vial of cells 91091815-1VL
生长模式
Adherent
核型
Modal no. 47
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
metastasis
运输
dry ice
储存温度
−196°C
应用
NCI-H292 has been used to determine the ability of the cell line to support the replication of mumps virus.
生化/生理作用
Derived from a cervical node metastasis of a pulmonary mucoepidermoid carcinoma from a 32 year old female. Cells retain mucoepidermoid characteristics in culture and support replication of Hepatitis B virus. Some of the cells contain numerous small mucin-containing granules and are L-DOPA decaroxylase negative. This cell line has been selected as a prototype for transfecting subgenome fragments into human cells for studying the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer. The cells are mucicarmine positive and also stain positively for keratin and vimentin but negative for neurofilament triplet protein. Ethnicity: Black.
Human lung, mucoepidermoid carcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 9,13
D5S818: 13
D7S820: 10
THO1: 8
TPOX: 8,11
vWA: 16,17
CSF1PO: 10
D13S317: 11,12
D16S539: 9,13
D5S818: 13
D7S820: 10
THO1: 8
TPOX: 8,11
vWA: 16,17
制备说明
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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