biological source
hamster ovary
manufacturer/tradename
ECACC
packaging
tube of 5 μg 94060607-DNA-5UG, pkg of vial of cells 94060607-1VL
growth mode
Adherent
karyotype
Modal no. 20
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
CHO/dhFr- has been used to study the relation between local chromatin mobility and transcriptional activity. It has also been used as a transfection lineage and also as a negative control in cytofluorometry experiments.
Biochem/physiol Actions
Hamster Chinese ovary
The CHO/dhFr- cell line lacks the enzyme dihydrofolate reductase (DHFR) which is necessary for purine synthesis. In the absence of exogenous purines, this enzyme is required for growth. Hence the CHO/dhFr- cell line has growth requirements for hypoxanthine (or adenine), glycine and thymidine. However, the cells will continue to grow without the addition of hypoxanthine and thymidine because the cells can obtain sufficient from the medium. α MEM minus does not contain the ribonucleosides or the deoxyribonucleosides, and will not support the growth of CHO/dhFr- or its serum free adaptation as neither contains the gene to produce DHFR. (See attached data sheet).
Preparation Note
IDMEM + 2mM Glutamine + 0.1mM Hypoxanthine + 0.01mM Thymidine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.