CHO/dhFr-

CHO/dhFr- has been used to study the relation between local chromatin mobility and transcriptional activity. It has also been used as a transfection lineage and also as a negative control in cytofluorometry experiments.

Hamster Chinese ovary

The CHO/dhFr- cell line lacks the enzyme dihydrofolate reductase (DHFR) which is necessary for purine synthesis. In the absence of exogenous purines, this enzyme is required for growth. Hence the CHO/dhFr- cell line has growth requirements for hypoxanthine (or adenine), glycine and thymidine. However, the cells will continue to grow without the addition of hypoxanthine and thymidine because the cells can obtain sufficient from the medium. α MEM minus does not contain the ribonucleosides or the deoxyribonucleosides, and will not support the growth of CHO/dhFr- or its serum free adaptation as neither contains the gene to produce DHFR. (See attached data sheet).

IDMEM + 2mM Glutamine + 0.1mM Hypoxanthine + 0.01mM Thymidine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C

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