等级
Molecular Biology
表单
buffered aqueous solution
运输
wet ice
储存温度
−20°C
一般描述
The Mammalian Amino-Terminal FLAG Stable Expression Kit provides the specialized FLAG® components needed for expression of a recombinant N-terminal FLAG fusion in mammalian cells. The pFLAG-CMV-3 and pFLAG-CMV-4 expression vectors encode the aminoglycoside phosphotransferase II gene (Neo) which confers resistance to aminoglycosides such as G418, allowing for selection of stable transfectants.
Vector Maps and Sequences
Vector Maps and Sequences
应用
The pFLAG-CMV-3 and pFLAG-CMV-4 Expression Vectors are suitable for transient or stable expression of secreted or cytoplasmic N-terminal FLAG® fusion proteins.
组分
- pFLAG-CMV-3a Expression Vector (E8770) 20 μg
- pFLAG-CMV-4a Expression Vector (E1775) 20 μg
- pFLAG-CMV-3-BAPa Control Plasmid (C3972) 20 μg
- pFLAG-CMV-4-BAPa Control Plasmid (C4722) 20 μg
- C-CMV-24 Reverse Sequencing Primer (P5475) 1 μg
- N-CMV-30 Forward Sequencing Primer (P5350) 1 μg
- ANTI-FLAG M2 Monoclonal Antibody (F3165) 200 μg
- ANTI-FLAG M5 Monoclonal Antibody (F4042) 200 μg
- ANTI-FLAG M2 Affinity Gel - Freezer Safe (A2220) 1 ml
- Met-FLAG-BAP Control Protein (P5975) 100 ug
- FLAG Peptide (F3290) 4 mg
原理
The FLAG® system is based on the FLAG marker octapeptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys). The FLAG sequence is hydrophilic and the last 5 amino acids (Asp-Asp-Asp-Asp-Lys) represent the target sequence of the protease enterokinase. FLAG fusion proteins are created by cloning the coding sequence of a protein of interest adjacent to the FLAG coding sequence in a FLAG expression vector, followed by expression in the appropriate host cell type.
法律信息
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持