grade
Molecular Biology
manufacturer/tradename
Lucigen RNR07250
packaging
pkg of 250 Units, 20U/µL
shipped in
dry ice
storage temp.
−20°C
General description
Ribonuclease R (RNase R) from E. coli is a magnesium-dependent 3′→ 5′ exoribonuclease that digests linear RNAs. RNase R does not digest lariat or circular RNA structures.1,2 Most cellular RNAs will be digested completely, except for tRNAs, 5S RNA, and intron lariats.
Lariats are produced during pre-mRNA splicing of intron regions (Figure1) and the 3´ tails will be trimmed by RNase R to the branch point nucleotide, where there is a 2´,5´-phosphodiester linkage.
Lariats are produced during pre-mRNA splicing of intron regions (Figure1) and the 3´ tails will be trimmed by RNase R to the branch point nucleotide, where there is a 2´,5´-phosphodiester linkage.
Application
- Removal of precursor linear RNA after circularisation of RNA for enhanced protein production
- Alternative splicing studies
- Gene expression studies
- Intron cDNA production
- Intronic screening of cDNA libraries
- Isolation of splicing intermediates and lariats
Features and Benefits
- Efficacy and quality assured under ISO 13485 certification.
- Available in bulk and for custom configurations.
- Effectively degrades linear RNA, Y-structure RNAs, but not lariat loops or circular RNAs.
- Digests linear RNAs to enrich for circular RNAs used for protein production or intronic cDNA library construction.
Note: RNase R requires low (0.1-1.0 mM) magnesium concentrations for activity. Low EDTA concentrations in substrate RNA solutions can negatively affect RNase R activity. Additional MgCl2 up to 1 mM final concentration can be used to compensate for EDTA in the substrate. Optimal activity is at 37 °C
signalword
Danger
hcodes
Hazard Classifications
ED ENV 1 - Met. Corr. 1
存储类别
10 - Combustible liquids
wgk
WGK 2
法规信息
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