SAE0150
Chondroitinase ABC from Proteus vulgaris
recombinant, low endotoxin, aqueous solution, ≥100 U/ml, 50-250 units/mg protein, BSA free
别名:
Ch′ase ABC, Chondroitin ABC Lyase
生物来源
bacterial (Proteus vulgaris)
质量水平
偶联物
(Glucosaminoglycan)
比活
50-250 units/mg protein
运输
dry ice
储存温度
−70°C
一般描述
Chondroitinase ABC is a lyase with specificity toward chondroitin sulfates and dermatan sulfates - with some minor activity toward hyaluronan. This recombinant version only contains the endolytic chondroitinase, meaning it will not digest the non-reducing ends of proteoglycan substrates. It targets β-(1,4)-linkages between N-acetylgalactosamine and either D-glucuronic acid or L-iduronic acid. The product of digestion is a mixture of Δ 4-unsaturated tetra- and di-saccharides.
应用
- Degradation of Chondroitin sulfate-containing tissue such as extracellular matrices and cartilage
- Neural regeneration research
- Eliminative degradation of all forms of Chondroitin sulfate: A, B and C
- Quantitation of glucosaminoglycans (mucopolysaccharides)
特点和优势
- Low Endotoxin
- BSA Free
- Recombinant
- Endolytic component only, does not contain exolytic enzyme.
- Compatible with chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate, and acts slowly on hyaluronate
制备说明
Store the product at -70 °C. When stored unopened at -70 °C, the buffer is stable for at least two years.
其他说明
1 unit will liberate 1.0 μmole of unsaturated disaccharides per min at pH 8.0 at 37 °C, using chondroitin sulfate from shark cartilage (C4384) as substrate.
Non active Ingredients: Hepes-Sodium salt, NaCl, Trehalose, Imidazole, Glycerol, Water
This product is for R&D use only. Not for drug, household, or other uses. Please consult the SafetyData Sheet for information regarding hazards and safe handling practices.
pH optimum: pH 8.0 (chondroitin sulfate)pH 6.8 (hyaluronic acid)
Temperature optimum: 37 °C
Activator: 0.05 M acetate
Inhibitors: 1 mM Zn2+ Ni2+, Fe2+ and Cu2+
Temperature optimum: 37 °C
Activator: 0.05 M acetate
Inhibitors: 1 mM Zn2+ Ni2+, Fe2+ and Cu2+
储存分类代码
10 - Combustible liquids
WGK
WGK 2
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Two distinct chondroitin sulfate ABC lyases an endoeliminase yielding tetrasaccharides and an exoeliminase preferentially acting on oligosaccharides
Hamai, A. et al.
The Journal of Biological Chemistry, 9123-9130 (1997)
Functions of Chondroitin Sulfate and Heparan Sulfate in the Developing Brain
Maeda, N., et al.
Neurochemical Research, 1228-1240 (2011)
商品
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
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