SRP4858
Heat Shock Protein 90 human
recombinant, expressed in baculovirus infected Sf9 cells, ≥99% (SDS-PAGE)
别名:
HSP86, HSPC1, HSPCA, HSPN, Hsp89, LAP2
生物来源
human
重组
expressed in baculovirus infected Sf9 cells
方案
≥99% (SDS-PAGE)
表单
liquid
分子量
~90 kDa
包装
pkg of 25 μg
储存条件
avoid repeated freeze/thaw cycles
杂质
endotoxin, tested
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... HSP90B1(7184)
一般描述
Heat shock protein 90 (HSP90) is a molecular chaperone and one of the most abundant proteins in unstressed cells. In mammalian cells, there are two genes encoding cytosolic Hsp90 homologues, with the human Hsp90α showing 85% sequence identity to Hsp90β. The recombinant Hsp90 produced from Sf9 cells represents the full length (a.a. 1-723) of Hsp90β subunit.
生化/生理作用
Heat shock protein 90kDa beta member 1 (HSP90B1) is activated during apoptosis. It is associated with the endoplasmic reticulum membrane and undergoes specific proteolytic cleavage leading to the activation of the caspase CPP32 and initiation of DNA fragmentation. The protein protects cells against flux in Ca2+ homeostasis, which would otherwise result in cell death. It maintains the chromosomal stability in mammalian cells by associating with FAC. HSP90B1 chaperones toll-like receptors (TLRs) and thus optimizes the B cell functioning. These proteins are also the main downstream chaperones which mediate the ER unfolded protein response (UPR), which is very essential for maintaining protein homeostasis in the ER.
外形
Protein is provided in a solution of 40 mM HEPES/KOH, pH 7.5, containing 400 mM potassium chloride and 5% glycerol.
制备说明
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
此项目有
R K Reddy et al.
The Journal of biological chemistry, 274(40), 28476-28483 (1999-09-25)
GRP94 is a 94-kDa chaperone glycoprotein with Ca(2+)-binding properties. We report here that during apoptosis induced by the topoisomerase II inhibitor etoposide, a fraction of GRP94 associated with the endoplasmic reticulum membrane undergoes specific proteolytic cleavage, coinciding with the activation
Bei Liu et al.
Blood, 112(4), 1223-1230 (2008-05-30)
Endoplasmic reticulum (ER) unfolded protein response (UPR) plays pivotal roles in both early B-cell development and plasma cell differentiation. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 (grp94, gp96) has
T Hoshino et al.
Blood, 91(11), 4379-4386 (1998-05-30)
The FAC protein encoded by the gene defective in Fanconi anemia (FA) complementation group C binds to at least three ubiquitous cytoplasmic proteins in vitro. We used here the complete coding sequence of FAC in a yeast two-hybrid screen to
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