melted. Cool the agarose to 45 °C. Add
1-2 units (1 unit for gels in 1X TAE, 2 units for gels in
1X TBE) agarase I per 100 mg of 1% agarose gel.
Incubate for 1 hr
room temperature.
8. Attach the Lysate Filter to a 45 mm neck bottle
(≥500 mL).
9. Add lysate to assembled Lysate Filter and apply
vacuum to clear cell debris.
10. Add 100 mL Binding Solution
hybridize 30-45
min at 37-50 °C.
6. Wash microplate wells 5X with 300 µl PBS−T.
7. Add 150 µl diluted anti-fluorescein-HRP conjugate
to well and incubate 30-45 min at 37°C.
8. Wash microplate
melted. Cool the agarose to 45 °C. Add
1-2 units (1 unit for gels in 1X TAE, 2 units for gels in
1X TBE) agarase I per 100 mg of 1% agarose gel.
Incubate for 1 hr
melted. Cool the agarose to 45 °C. Add
1-2 units (1 unit for gels in 1X TAE, 2 units for gels in
1X TBE) agarase I per 100 mg of 1% agarose gel.
Incubate for 1 hr
melted. Cool the agarose to 45 °C. Add
1-2 units (1 unit for gels in 1X TAE, 2 units for gels in
1X TBE) agarase I per 100 mg of 1% agarose gel.
Incubate for 1 hr